Literature DB >> 5575571

Role of sodium carboxymethyl cellulose and hydroxyethyl starch in hematopoietic cell line cultures.

A Mizrahi, G E Moore.   

Abstract

Sodium carboxymethyl cellulose and hydroxyethyl starch were found to support rapid growth of two hematopoietic cell lines. The polymers were not metabolized by the cells. In the presence of these compounds, lower rates of glucose utilization and lactic acid production were observed. The uptake of glucose by the cells decreased as the concentration of the polymer in the medium was increased. These results indicate that sodium carboxymethyl cellulose and hydroxyethyl starch probably protect the cells against physical stress in suspended cultures.

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Year:  1971        PMID： 5575571      PMCID： PMC377270          DOI： 10.1128/am.21.4.754-757.1971

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  8 in total

1.  AUTOMATED LACTIC ACID DETERMINATION IN SERUM AND TISSUE EXTRACTS.

Authors:  N J HOCHELLA; S WEINHOUSE
Journal:  Anal Biochem       Date:  1965-02       Impact factor: 3.365

2.  A chemically defined medium for growth of animal cells in suspension.

Authors:  S C NAGLE; H R TRIBBLE; R E ANDERSON; N D GARY
Journal:  Proc Soc Exp Biol Med       Date:  1963-02

3.  Massive fluid-suspension cultures of certain mammalian tissue cells. II. Glucose utilization and cell proliferation.

Authors:  J C BRYANT; E L SCHILLING; W R EARLE
Journal:  J Natl Cancer Inst       Date:  1958-08       Impact factor: 13.506

4.  Culture of normal human leukocytes.

Authors:  G E Moore; R E Gerner; H A Franklin
Journal:  JAMA       Date:  1967-02-20       Impact factor: 56.272

5.  Methylcellulose effect on cell proliferation and glucose utilization in chemically defined medium in large stationary cultures.

Authors:  J C Bryant
Journal:  Biotechnol Bioeng       Date:  1969-03       Impact factor: 4.530

6.  An improved chemically defined culture medium for strain L mouse cells based on growth responses to graded levels of nutrients including iron and zinc ions.

Authors:  K Higuchi
Journal:  J Cell Physiol       Date:  1970-02       Impact factor: 6.384

7.  Mammalian cells in chemically defined media in suspension cultures.

Authors:  J C Bryant
Journal:  Ann N Y Acad Sci       Date:  1966-10-07       Impact factor: 5.691

8.  Partial substitution of serum in hematopoietic cell line media by synthetic polymers.

Authors:  A Mizrahi; G E Moore
Journal:  Appl Microbiol       Date:  1970-06
  8 in total

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