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Literature DB >> 5575565

Free and immobilized glucose isomerase from Streptomyces phaeochromogenes.

Abstract

Properties were determined of the glucose isomerase from Streptomyces phaeochromogenes NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a pH optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum pH were studied. Michaelis constants for glucose and Mg(2+) were 0.25 and 0.025 m, respectively. Co(2+) enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.

Entities: Chemical Disease Species

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Year: 1971 PMID： 5575565 PMCID： PMC377236 DOI： 10.1128/am.21.4.588-593.1971

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

9 in total

1. STATISTICAL METHODS AND CONTROL IN BACTERIOLOGY.

Authors: C Eisenhart; P W Wilson
Journal: Bacteriol Rev Date: 1943-06

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. Water-insoluble enzymes: arrangement of aldolase within an insoluble carrier.

Authors: P Bernfeld; R E Bieber; P C MacDonnell
Journal: Arch Biochem Biophys Date: 1968-09-20 Impact factor: 4.013

4. Lattice entrapment of glycolytic enzymes.

Authors: H D Brown; A B Patel; S K Chattopadhyay
Journal: J Chromatogr Date: 1968-05-21

5. Automated method to determine D-fructose in the presence of D-glucose.

Authors: M C Cadmus; G W Strandberg
Journal: Anal Biochem Date: 1968-12 Impact factor: 3.365

6. The preparation and characterization of lyophilized polyacrylamide enzyme gels for chemical analysis.

Authors: G P Hicks; S J Updike
Journal: Anal Chem Date: 1966-05 Impact factor: 6.986

7. Reagentless substrate analysis with immobilized enzymes.

Authors: S J Updike; G P Hicks
Journal: Science Date: 1967-10-13 Impact factor: 47.728

8. Entrapment of enzymes and microorganisms in synthetic cross-linked polymers and their application in column techniques.

Authors: K Mosbach; R Mosbach
Journal: Acta Chem Scand Date: 1966

8. Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions.

Authors: Riyaz Zafar Sayyed; G B Shimpi; S B Chincholkar
Journal: J Food Sci Technol Date: 2010-10-19 Impact factor: 2.701

9. Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1.

Authors: Sheetal Bhasin; H A Modi
Journal: Biotechnol Res Int Date: 2012-07-26

9 in total

Free and immobilized glucose isomerase from Streptomyces phaeochromogenes.

1. STATISTICAL METHODS AND CONTROL IN BACTERIOLOGY.

2. Protein measurement with the Folin phenol reagent.

3. Water-insoluble enzymes: arrangement of aldolase within an insoluble carrier.

4. Lattice entrapment of glycolytic enzymes.

5. Automated method to determine D-fructose in the presence of D-glucose.

6. The preparation and characterization of lyophilized polyacrylamide enzyme gels for chemical analysis.

7. Reagentless substrate analysis with immobilized enzymes.

8. Entrapment of enzymes and microorganisms in synthetic cross-linked polymers and their application in column techniques.

9. ANTIGENS AND ENZYMES MADE INSOLUBLE BY ENTRAPPING THEM INTO LATTICES OF SYNTHETIC POLYMERS.

1. Immobilization of Glucose Isomerase-Containing Streptomyces phaeochromogenes Cells in Fine-Particle Form.

2. Purification, Immobilization, and Some Properties of Glucose Isomerase from Streptomyces flavogriseus.

3. Production of Glucose Isomerase by Streptomyces flavogriseus.

4. Studies on glucose isomerase from a Streptomyces species.

5. Extraction of glucose isomerase from Streptomyces flavogriseus.

6. Properties of D-xylose isomerase from Streptomyces albus.

7. D-Glucose isomerase: constitutive and catabolite repression-resistant mutants of Streptomyces phaeochromogenes.

8. Constitutive production of extracellular glucose isomerase by an osmophillic Aspergillus sp. under submerged conditions.

9. Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1.