Literature DB >> 5574518

Salt extraction of soluble HL-A antigens.

R A Reisfeld, M A Pellegrino, B D Kahan.   

Abstract

Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.

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Year:  1971        PMID： 5574518     DOI： 10.1126/science.172.3988.1134

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  38 in total

1.  Utilization of cultured human lymphoid cells for detection of humoral sensitization in prospective recipients of kidney transplants.

Authors:  S Ferrone; A Ting; M A Pellegrino; P I Terasaki; R A Reisfeld
Journal:  J Clin Invest       Date:  1975-02       Impact factor: 14.808

2.  A molecular defect in thrombasthenic platelets.

Authors:  L Degos; A Dautigny; J C Brouet; M Colombani; N Ardaillou; J P Caen; J Colombani
Journal:  J Clin Invest       Date:  1975-07       Impact factor: 14.808

3.  The topographical location and unique nature of a glucokinase associated with the Golgi apparatus of rat liver.

Authors:  G Berthillier; R Coleman; D G Walker
Journal:  Biochem J       Date:  1976-01-15       Impact factor: 3.857

4.  Relationship between general or specific immunoreactivity and prognosis in postoperative patients with hepatocellular carcinoma.

Authors:  H Yamasaki; E Okamoto; T Komatsu; Y Manabe; S Shinka
Journal:  Cancer Immunol Immunother       Date:  1990       Impact factor: 6.968

5.  Further characterization of a human T lymphocyte associated antigen.

Authors:  J K Anderson; R S Metzgar
Journal:  Clin Exp Immunol       Date:  1979-08       Impact factor: 4.330

6.  Virus-receptor interaction in the adenovirus system I. Identification of virion attachment proteins of the HeLa cell plasma membrane.

Authors:  U Svensson; R Persson; E Everitt
Journal:  J Virol       Date:  1981-04       Impact factor: 5.103

7.  Interaction of purified leukocidin from Pseudomonas aeruginosa with Bovine polymorphonuclear leukocytes.

Authors:  W Scharmann
Journal:  Infect Immun       Date:  1976-04       Impact factor: 3.441

8.  Tumor-reactive IgE antibodies in plasma of patients with gastrointestinal carcinomas.

Authors:  I Berczi; V Holford-Strevens; Z H Warsi; L S McMorris; R H Thorlakson; T K Thorlakson; A H Sehon
Journal:  Cancer Immunol Immunother       Date:  1983       Impact factor: 6.968

9.  [Structure and function of virus-induced antigens in cultured cells infected with Merek's disease and turkey herpes viruses. II. Isolation of intracellular antigens from infected cells (author's transl)].

Authors:  O R Kaaden
Journal:  Med Microbiol Immunol       Date:  1977-10-07       Impact factor: 3.402

10.  The use of 1-fluoro-2,4-dinitrobenzene as an affinity label for the antigen receptor of delayed hypersensitivity.

Authors:  H P Godfrey
Journal:  Immunology       Date:  1976-10       Impact factor: 7.397
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