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Literature DB >> 5571840

The preparation of 59 Fe-labelled transferrin for ferrokinetic studies.

Abstract

Serum labelled with (59)Fe for ferrokinetic studies should contain only [(59)Fe] transferrin. [(59)Fe] ferric citrate was incubated at 37 degrees C for up to 24 hours with sera having an excess latent iron-binding capacity. The results showed that unbound [(59)Fe] ferric citrate may be present for several hours. An anion exchange resin (IRA 400 Cl(-)) column removed all the [(59)Fe] ferric citrate and left only [(59)Fe] transferrin in the eluate. It is suggested that all (59)Fe-labelled preparations for ferrokinetic studies should be passed through a resin column before injection.

Entities: Chemical Gene

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Year: 1971 PMID： 5571840 PMCID： PMC477030 DOI： 10.1136/jcp.24.5.472

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

7 in total

1. THE SPECIFIC BINDING OF IRON(III) AND COPPER(II) TO TRANSFERRIN AND CONALBUMIN.

Authors: R AASA; B G MALMSTROEM; P SALTMAN
Journal: Biochim Biophys Acta Date: 1963-09-24

Review 2. Ferrokinetics in man.

Authors: C A Finch; K Deubelbeiss; J D Cook; J W Eschbach; L A Harker; D D Funk; G Marsaglia; R S Hillman; S Slichter; J W Adamson; A Ganzoni; E R Biblett
Journal: Medicine (Baltimore) Date: 1970-01 Impact factor: 1.889

3. The kinetics and mechanism of iron (3) exchange between chelates and transferrin. II. The presentation and removal with ethylenediaminetetraacetate.

Authors: G W Bates; C Billups; P Saltman
Journal: J Biol Chem Date: 1967-06-25 Impact factor: 5.157

4. Citrate-mediated exchange of FE3+ among tranferrin molecules.

Authors: P Aisen
Journal: Biochem Biophys Res Commun Date: 1968-07-26 Impact factor: 3.575

5. Skin clearance of iron in normal and iron deficient subjects.

Authors: I Cavill; A Jacobs
Journal: Br J Dermatol Date: 1970-02 Impact factor: 9.302

6. The transport of iron by rat intestine.

Authors: J H Helbock; P Saltman
Journal: Biochim Biophys Acta Date: 1967

7. METHOD FOR THE AUTOMATIC DETERMINATION OF SERUM IRON.

Authors: D S YOUNG; J M HICKS
Journal: J Clin Pathol Date: 1965-01 Impact factor: 3.411

7 in total

16 in total

1. The mammalian neuroendocrine hormone norepinephrine supplies iron for bacterial growth in the presence of transferrin or lactoferrin.

Authors: P P Freestone; M Lyte; C P Neal; A F Maggs; R D Haigh; P H Williams
Journal: J Bacteriol Date: 2000-11 Impact factor: 3.490

2. Uptake and intracellular distribution of iron from transferrin and chelators in erythroid cells.

Authors: G J Kontoghiorghes; A May
Journal: Biol Met Date: 1990

3. Measurement of plasma volume using 59Fe-labelled transferrin.

Authors: C Ricketts; I Cavill
Journal: J Clin Pathol Date: 1978-02 Impact factor: 3.411

4. Chelation of transferrin iron by desferrioxamine in K562 cells. The partition of iron between ferrioxamine and ferritin.

Authors: S Roberts; A Bomford
Journal: Biochem J Date: 1988-09-15 Impact factor: 3.857

The preparation of 59 Fe-labelled transferrin for ferrokinetic studies.

1. THE SPECIFIC BINDING OF IRON(III) AND COPPER(II) TO TRANSFERRIN AND CONALBUMIN.

Review 2. Ferrokinetics in man.

3. The kinetics and mechanism of iron (3) exchange between chelates and transferrin. II. The presentation and removal with ethylenediaminetetraacetate.

4. Citrate-mediated exchange of FE3+ among tranferrin molecules.

5. Skin clearance of iron in normal and iron deficient subjects.

6. The transport of iron by rat intestine.

7. METHOD FOR THE AUTOMATIC DETERMINATION OF SERUM IRON.

1. The mammalian neuroendocrine hormone norepinephrine supplies iron for bacterial growth in the presence of transferrin or lactoferrin.

2. Uptake and intracellular distribution of iron from transferrin and chelators in erythroid cells.

3. Measurement of plasma volume using 59Fe-labelled transferrin.

4. Chelation of transferrin iron by desferrioxamine in K562 cells. The partition of iron between ferrioxamine and ferritin.

5. [The plasma 59Fe clearance curve in man. An evaluation of methods of measurement and analysis].

6. Transferrin iron uptake by human synovium.

7. The use of Chang cells cultured in vitro for the investigation of cellular iron metabolism.

8. The effect of cobalt on the uptake of plasma iron by the liver.

9. The effect of desferrioxamine on transferrin receptors, the cell cycle and growth rates of human leukaemic cells.

10. Systems analysis of iron metabolism: the network of iron pools and fluxes.