Literature DB >> 5550501

Cell surface antigens: serial sectioning of single cells as an approach to topographical analysis.

C W Stackpole, T Aoki, E A Boyse, L J Old, J Lumley-Frank, E De Harven.   

Abstract

The topographical distribution of H-2 antigens on the surfaces of C57BL/6 mouse thymocytes and lymph-node lymphocytes was investigated by a new technique. Single cells were reacted with visually labeled antibody directed against H-2 antigen. Each cell was processed for electron microscopy individually and was serially sectioned. Models constructed from serial electron micrographs provided detailed views of the entire cell surface and showed that H-2 antigen occurs on thymocytes in small isolated regions and on lymphocytes in large interconnected regions.

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Year:  1971        PMID: 5550501     DOI: 10.1126/science.172.3982.472

Source DB:  PubMed          Journal:  Science        ISSN: 0036-8075            Impact factor:   47.728


  15 in total

1.  Hybrid antibody-induced topographical redistribution of surface immunoglobulins, alloantigens, and concanavalin A receptors on mouse lymphoid cells.

Authors:  C W Stackpole; L T De Milio; U Hämmerling; J B Jacobson; M P Lardis
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205

2.  [Observations on special structures of the nuclear membrane by the freeze-etching technique].

Authors:  H W Meyer; J Roth; F Bolck
Journal:  Protoplasma       Date:  1972       Impact factor: 3.356

3.  Ultrastructural localization of human HL-A membrane antigens by use of hybrid antibodies.

Authors:  C Neauport-Sautes; D Silvestre; M G Niccolai; F M Kourilsky; J P Levy
Journal:  Immunology       Date:  1972-05       Impact factor: 7.397

4.  [The arrangement of membrane proteins. Studies of erythrocyte-ghosts by freeze-etching].

Authors:  H W Meyer; H Winkelmann
Journal:  Protoplasma       Date:  1972       Impact factor: 3.356

Review 5.  Selective gene action in the specification of cell surface structure.

Authors:  E A Boyse; L J Old; M Scheid
Journal:  Am J Pathol       Date:  1971-11       Impact factor: 4.307

6.  Involvement of spectrin in cell-surface receptor capping in lymphocytes.

Authors:  W J Nelson; C A Colaço; E Lazarides
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

7.  Role of a pertussis toxin substrate in the control of lectin-induced cap formation in human neutrophils.

Authors:  P M Lad; C V Olson; I S Grewal
Journal:  Biochem J       Date:  1986-08-15       Impact factor: 3.857

8.  The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles.

Authors:  T W Tillack; R E Scott; V T Marchesi
Journal:  J Exp Med       Date:  1972-06-01       Impact factor: 14.307

9.  Ligand-induced movement of lymphocyte membrane macromolecules. II. Mapping of surface moieties.

Authors:  M J Karnovsky; E R Unanue; M Leventhal
Journal:  J Exp Med       Date:  1972-10-01       Impact factor: 14.307

Review 10.  Plasma membranes of mammalian cells: a review of methods for their characterization and isolation.

Authors:  J W DePierre; M L Karnovsky
Journal:  J Cell Biol       Date:  1973-02       Impact factor: 10.539

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