Literature DB >> 5541003

Purified protoplasmic peptides of mycobacteria: chemical composition of a tuberculin-active glycopeptide.

K D Stottmeier, R E Beam, H L David, R Shapira, D C Farshy.   

Abstract

A tuberculin-active glycopeptide containing eight different amino acids and glucose was isolated from the protoplasm of Mycobacterium tuberculosis. A molecular weight of 4,000 to 5,000 was established by Sephadex gel filtration; other analyses showed a peptide to carbohydrate ratio of 9:1. These observations suggest a tentative composition of 3 to 4 residues of glucose, 12 residues each of aspartic and glutamic acids, 3 residues each of lysine, glycine, and serine, and 1 residue each of arginine, threonine, and alanine.

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Year:  1971        PMID： 5541003      PMCID： PMC248338          DOI： 10.1128/jb.105.1.172-175.1971

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  Phosphorus assay in column chromatography.

Authors:  G R BARTLETT
Journal:  J Biol Chem       Date:  1959-03       Impact factor: 5.157

2.  The immunology and chemistry of tuberculin. I. The isolation of dialyzable and nondialyzable tuberculin-active components from unheated BCG culture filtrates.

Authors:  H BAER; S D CHAPARAS
Journal:  Am Rev Respir Dis       Date:  1963-07

3.  Purified protoplasmic peptides of mycobacteria: isolation of species-specific peptides from protoplasm of mycobacteria.

Authors:  R E Beam; K D Stottmeier; G P Kubica
Journal:  J Bacteriol       Date:  1969-10       Impact factor: 3.490

4.  [Analysis by polyacrylamide gel isoelectric focusing of protein fractions present in the purified protein derivatives (PPD)].

Authors:  J Augier; S Augier-Gibory
Journal:  Ann Inst Pasteur (Paris)       Date:  1969-12

5.  [Electrophoretic bidimensional analysis in polyacrylamide gel of the tuberculoproteins present in IP and in various other preparations of PPD].

Authors:  J Augier; S Augier-Gibory
Journal:  Ann Inst Pasteur (Paris)       Date:  1969-05

6.  Tetracyanoethylated pentaerythritol: an efficient polar liquid phase for analysis of trimethylsilyl derivatives of sugars and sugar alcohols.

Authors:  D Farshtchi; C W Moss
Journal:  J Chromatogr       Date:  1969-06-03

7.  Characterization of bacteria by gas chromatography: comparison of trimethylsilyl derivatives of whole-cell hydrolysates.

Authors:  D Farshtchi; C W Moss
Journal:  Appl Microbiol       Date:  1969-02

8.  Purified protoplasmic peptides of mycobacteria: in vivo and in vitro comparison of the species specificity of purified protoplasmic peptides and purified protein derivatives of mycobacterial culture filtrates.

Authors:  K D Stottmeier; R E Beam; G P Kubica
Journal:  J Bacteriol       Date:  1969-10       Impact factor: 3.490

9.  Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors:  P Andrews
Journal:  Biochem J       Date:  1964-05       Impact factor: 3.766
  9 in total
  1 in total

Review 1.  Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

Authors:  T M Daniel; B W Janicki
Journal:  Microbiol Rev       Date:  1978-03
  1 in total

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