Literature DB >> 55405

Glycoproteins in membranes of secretory granules of the anterior pituitary gland.

J B Warchol, D C Herbert, E G Rennels.   

Abstract

Rat anterior pituitary glands were examined by electron microscopy after staining with five different histochemical stains. Histochemical reactions were observed in the cell coat, cell membrane and the membrane surrounding the secretory granules in all anterior pituitary cells following staining with phosphotungstic acid (PTA), chromic acid and PTA, the periodic acid-thiosemicarbazide-silver protein method (PA-TSC-SP) of Thiéry, ruthenium red and concanavalin A. The staining was abolished when the sections were preincubated with pronase, neuraminidase or trypsin and subsequently exposed to PTA, chromic acid and PTA or PA-TSC-SP. The possible functional role of the glycoproteins present in the membrane surrounding the secretory granules is considered.

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Year:  1976        PMID： 55405     DOI： 10.1007/bf02462738

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  34 in total

1.  Specificity of phosphotungstic acid used as a section stain to visualize surface coats of cells.

Authors:  G B Dermer
Journal:  J Ultrastruct Res       Date:  1973-11

2.  Electron microscopic localization of glycoproteins in pituitary cells of duck and quail.

Authors:  A Tixier-Vidal; R Picart
Journal:  J Histochem Cytochem       Date:  1971-12       Impact factor: 2.479

3.  Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action.

Authors:  J H Luft
Journal:  Anat Rec       Date:  1971-11

4.  [Identification by electron microscopy of six cellular types in the antehypophysis of the rat with the aid of a coloration technic using chromic-phosphotungstic acid mixture].

Authors:  A Rambourg; J Racadot
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1968-01-08

5.  Comparative analysis of zymogen granule membrane polypeptides.

Authors:  R J MacDonald; R A Ronzio
Journal:  Biochem Biophys Res Commun       Date:  1972-10-17       Impact factor: 3.575

6.  Protein-carbonhydrate interaction. 3. Agar gel-diffusion studies on the interaction of Concanavalin A, a lectin isolated from jack bean, with polysaccharides.

Authors:  I J Goldstein; L L So
Journal:  Arch Biochem Biophys       Date:  1965-08       Impact factor: 4.013

7.  The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion.

Authors:  S Roseman
Journal:  Chem Phys Lipids       Date:  1970-10       Impact factor: 3.329

8.  [Functional significance of a cell category of the human fetal antehypophysis].

Authors:  P Dubois
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1971-09-06

9.  Fine structures of capillary and endocapillary layer as revealed by ruthenium red.

Authors:  J H Luft
Journal:  Fed Proc       Date:  1966 Nov-Dec

10.  The effect of neuraminidase (3:2:1:18) on the aggregation of cells dissociated from embryonic chick muscle tissue.

Authors:  R B Kemp
Journal:  J Cell Sci       Date:  1970-05       Impact factor: 5.285
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  2 in total

1.  Cytochemical duality of neurosecretory material in the hypothalamo-posthypophysial system of the rat as related to hormonal content.

Authors:  F Tasso; S Rua; D Picard
Journal:  Cell Tissue Res       Date:  1977-05-10       Impact factor: 5.249

2.  Studies on the ultrastructure of the rotifer Habrotrocha rosa Donner (Aschelminthes). The alimentary system.

Authors:  U Schramm
Journal:  Cell Tissue Res       Date:  1978-06-08       Impact factor: 5.249
  2 in total

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