The potential distribution and the short-circuiting factor in the sucrose gap.

The sucrose gap technique, though widely employed in many tissues, could not be used for quantitative measurements of the membrane potential, because the value of the short-circuiting factor and the influence of junction potential on the recorded potential difference were unknown. The formula that relates the recorded potential to the true resting membrane potential was found by application of the cable equations to a core conductor placed in a system with three different media, e.g. Ringer, sucrose, and KCl. The formula shows that the potential difference recorded over the sucrose insulator depends on the extracellular and the intracellular longitudinal resistances, the membrane resistance and the membrane potentials in each region, and on the junction potentials between the different media. The true membrane potential in the Ringer region can be calculated from the potential difference recorded after complete depolarization by KCl on one side of the preparation, if the longitudinal resistances, the membrane resistances, the extracellular potential in the sucrose, and the junction potential between Ringer and sucrose are determined by separate measurements.