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Literature DB >> 5528638

Purification and crystallization of avidin.

Abstract

An improved purification, including crystallization, of avidin from hen's-egg white is described. The product bound 15.1mug of biotin/mg, corresponding to an equivalent weight of 16200. The amino acid composition showed an integral number of amino acid residues per 16200g, which suggested that each molecule of avidin (mol. wt. approx. 66000) contained four identical subunits.

Entities: Chemical

Mesh：

Substances：

Year: 1970 PMID： 5528638 PMCID： PMC1179080 DOI： 10.1042/bj1180067

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

7 in total

1. AVIDIN. 2. PURIFICATION AND COMPOSITION.

Authors: M D MELAMED; N M GREEN
Journal: Biochem J Date: 1963-12 Impact factor: 3.857

2. AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION.

Authors: M J CRUMPTON; J M WILKINSON
Journal: Biochem J Date: 1963-08 Impact factor: 3.857

3. Avidin. I. Isolation and characterization of the protein and nucleic acid.

Authors: H FRAENKEL-CONRAT; N S SNELL; E D DUCAY
Journal: Arch Biochem Biophys Date: 1952-07 Impact factor: 4.013

Review 4. [Malignant tumors in otorhinolaryngology. II. Tumors of the nose and its sinuses. 2. Treatment methods, results, problems of after care and rehabilitation].

Authors: W Kup; D Lange
Journal: Arch Geschwulstforsch Date: 1970

5. Evidence for a genetic relationship between avidins and lysozymes.

Authors: N M Green
Journal: Nature Date: 1968-01-20 Impact factor: 49.962

6. A preliminary crystallographic investigation of avidin.

Authors: N M Green; M A Joynson
Journal: Biochem J Date: 1970-06 Impact factor: 3.857

7. The effect of N-bromosuccinimide on the sub-unit structure of acidin and its complexes with biotin.

Authors: N M Green; M E Ross
Journal: Biochem J Date: 1968-11 Impact factor: 3.857

7 in total

11 in total

1. A preliminary crystallographic investigation of avidin.

Authors: N M Green; M A Joynson
Journal: Biochem J Date: 1970-06 Impact factor: 3.857

2. Molecular cloning of the chicken avidin cDNA.

Authors: M L Gope; R A Keinänen; P A Kristo; O M Conneely; W G Beattie; T Zarucki-Schulz; B W O'Malley; M S Kulomaa
Journal: Nucleic Acids Res Date: 1987-04-24 Impact factor: 16.971

3. A new method of in situ hybridization.

Authors: J E Manning; N D Hershey; T R Broker; M Pellegrini; H K Mitchell; N Davidson
Journal: Chromosoma Date: 1975-11-24 Impact factor: 4.316

4. The dissociation of avidin-biotin complexes by guanidinium chloride.

Authors: N M Green; E J Toms
Journal: Biochem J Date: 1972-12 Impact factor: 3.857

5. Pig liver pyruvate carboxylase. Purification, properties and cation specificity.

Authors: G B Warren; K F Tipton
Journal: Biochem J Date: 1974-05 Impact factor: 3.857

6. Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels.

Authors: T R Broker; L M Angerer; P H Yen; N D Hershey; N Davidson
Journal: Nucleic Acids Res Date: 1978-02 Impact factor: 16.971

7. The properties of subunits of avidin coupled to sepharose.

Authors: N M Green; E J Toms
Journal: Biochem J Date: 1973-08 Impact factor: 3.857

8. The use of bifunctional biotinyl compounds to determine the arrangement of subunits in avidin.

Authors: N M Green; L Konieczny; E J Toms; R C Valentine
Journal: Biochem J Date: 1971-12 Impact factor: 3.857

9. Role of human serum biotinidase as biotin-binding protein.

Authors: J Chauhan; K Dakshinamurti
Journal: Biochem J Date: 1988-11-15 Impact factor: 3.857

10. Indicators of marginal biotin deficiency and repletion in humans: validation of 3-hydroxyisovaleric acid excretion and a leucine challenge.

Authors: Donald M Mock; Cindy L Henrich; Nadine Carnell; Nell I Mock
Journal: Am J Clin Nutr Date: 2002-11 Impact factor: 7.045