A simple Beckman DU accessory for location of proteins separated by isoelectric focusing in granulated gel layers: isolation of human serum very low density apolipoproteins C-II and C-III-1.

A simple, low-cost gel layer scanner has been developed for the Beckman DU spectrophotometer. The gel scanner makes it possible to localize zones precisely after preparative isoelectric focusing of proteins in 2-mm thick Sephadex G-200 layers. Using the scanner after isoelectric separation of a mixture of human serum apolipoproteins C-III-1 and C-II, pure proteis could easily be obtained.