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Literature DB >> 5499967

Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum.

R A Cox, K Kanagalingam, K S Sutherland.

Abstract

1. RNA was isolated from virus-like particles found in Penicillium chrysogenum and resolved into two fractions by gel filtration through agarose columns. 2. Fraction 1 was excluded and had the following properties: 50.9% G+C [AMP 0.246, UMP 0.246, CMP 0.252, GMP 0.255 (mole fraction)]; mol.wt. about 1.2x10(6) daltons; s(20,w) 12.3S and ;melting' temperature about 100 degrees C (solvent 0.15m-sodium chloride-0.015m-sodium citrate pH7.2); optical rotation [alpha](max.) 6000 degrees at 278nm; circular dichroism (epsilon(L)-epsilon(R))(max.)=8.181mol(-1) cm(-1) at 260nm. 3. Properties of fraction 2 include 37.8% G+C [AMP 0.313, UMP 0.312, CMP 0.186, GMP 0.189 (mole fraction)]; mol.wt. about 140000 daltons; s(20,w) 7.3S, T(m) about 85 degrees C (solvent 0.15m-sodium chloride-0.015m-sodium citrate, pH7.2); optical rotation [alpha](max.) 6000 degrees at 278nm; circular dichroism (epsilon(L)-epsilon(R))(max.)=8.241mol(-1) cm(-1) at 260nm. 4. The properties of both fractions were consistent with a double-helical conformation.

Entities: Chemical Disease Species

Mesh：
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Substances：
Buffers

Year: 1970 PMID： 5499967 PMCID： PMC1179636 DOI： 10.1042/bj1200549

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

25 in total

1. The native, denatured and renatured states of deoxyribonucleic acid.

Authors: J Eigner; P Doty
Journal: J Mol Biol Date: 1965-07 Impact factor: 5.469

2. Optical rotatory dispersion of E. coli ribosomes and their constituents.

Authors: P K Sarkar; J T Yang; P Doty
Journal: Biopolymers Date: 1967-01 Impact factor: 2.505

3. Interferon stimulation by a double stranded RNA of a mycophage in statolon preparations.

Authors: W J Kleischmidt; L F Ellis; R M Van Frank; E B Murphy
Journal: Nature Date: 1968-10-12 Impact factor: 49.962

4. The secondary structure of ribosomal ribonucleic acid in solution.

Authors: R A Cox
Journal: Biochem J Date: 1966-03 Impact factor: 3.857

5. The optical rotatory dispersion of ribosomes and their constituents.

Authors: P McPhie; W B Gratzer
Journal: Biochemistry Date: 1966-04 Impact factor: 3.162

6. Inducers of interferon and host resistance. I. Double-stranded RNA from extracts of Penicillium funiculosum.

Authors: G P Lampson; A A Tytell; A K Field; M M Nemes; M R Hilleman
Journal: Proc Natl Acad Sci U S A Date: 1967-08 Impact factor: 11.205

7. Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes.

Authors: A K Field; A A Tytell; G P Lampson; M R Hilleman
Journal: Proc Natl Acad Sci U S A Date: 1967-09 Impact factor: 11.205

8. Biophysical studies on double-stranded RNA from turnip yellow mosaic virus-infected plants.

Authors: L E Bockstahler
Journal: Mol Gen Genet Date: 1967

9. A spectrophotometric study of the denaturation of deoxyribonucleic acid in the presence of urea or formaldehyde and its relevance to the secondary structure of single-stranded polynucleotides.

Authors: R A Cox; K Kanagalingam
Journal: Biochem J Date: 1968-07 Impact factor: 3.857

10. A possible method for characterizing the secondary structure of ribonucleic acids.

Authors: R A Cox
Journal: Biochem J Date: 1966-07 Impact factor: 3.857

9 in total

1. Calculation of the circular dichroism of bihelical ribonucleic acids as a function of nucleotide composition.

Authors: R A Cox; W Hirst
Journal: Biochem J Date: 1976-05-01 Impact factor: 3.857

Review 2. Fungal viruses.

Authors: P A Lemke; C H Nash
Journal: Bacteriol Rev Date: 1974-03

3. Conformation of nucleic acids and the analysis of the hypochromic effect.

Authors: R A Cox
Journal: Biochem J Date: 1970-12 Impact factor: 3.857

4. Spectroscopic evidence for the uneven distribution of adenine and uracil residues in ribosomal ribonucleic acid of Drosophila melanogaster and of Plasmodium knowlesi and its possible evolutionary significance.

Authors: R A Cox; E Godwin; J R Hastings
Journal: Biochem J Date: 1976-06-01 Impact factor: 3.857

5. Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gels.

Authors: S L Petrović; B D Sumonja; R B Vasiljević
Journal: Biochem J Date: 1974-04 Impact factor: 3.857

6. Thermal denaturation in acidic solutions of double-helical ribonucleic acid from virus-like particles found in Penicillium chrysogenum. A spectrophotometric study.

Authors: R A Cox; K Kanagalingam; E Sutherland
Journal: Biochem J Date: 1971-11 Impact factor: 3.857