Literature DB >> 5484679

Isoelectric focusing of human liver alkaline phosphatase.

A L Latner, M E Parsons, A W Skillen.   

Abstract

1. Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of human liver alkaline phosphatase was found to be pH3.9.

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Year:  1970        PMID： 5484679      PMCID： PMC1179117          DOI： 10.1042/bj1180299

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  HETEROGENEITY OF HUMAN INTESTINAL ALKALINE PHOSPHATASE.

Authors:  D W MOSS
Journal:  Nature       Date:  1963-12-21       Impact factor: 49.962

2.  Preparation and properties of highly purified alkaline phosphatase from swine kidneys.

Authors:  J C MATHIES
Journal:  J Biol Chem       Date:  1958-11       Impact factor: 5.157

3.  The Possible Significance of Hexosephosphoric Esters in Ossification: Part VII. The Bone Phosphatase.

Authors:  M Martland; R Robison
Journal:  Biochem J       Date:  1927       Impact factor: 3.857

4.  Properties of alkaline-phosphatase fractions separated by starch-gel electrophoresis.

Authors:  D W MOSS; E J KING
Journal:  Biochem J       Date:  1962-07       Impact factor: 3.857

5.  Alkaline phosphatase of Escherichia coli: a zinc metalloenzyme.

Authors:  D J PLOCKE; C LEVINTHAL; B L VALLEE
Journal:  Biochemistry       Date:  1962-05-25       Impact factor: 3.162

6.  Reversible inactivation of alkaline phosphatase in acid solution.

Authors:  P B Scutt; D W Moss
Journal:  Enzymologia       Date:  1968-09-30

7.  A simple apparatus and procedure for electrofocusing experiments: pL of lactate dehydrogenase and isocitrate lyase.

Authors:  D L Weller; A Heaney; R E Sjogren
Journal:  Biochim Biophys Acta       Date:  1968-12-03

8.  Isoelectric focusing and separation of the subcomponents of lactoperoxidase.

Authors:  A Carlström; O Vesterberg
Journal:  Acta Chem Scand       Date:  1967

9.  The reversible inactivation of pig kidney alkaline phosphatase at low pH.

Authors:  P J Butterworth
Journal:  Biochem J       Date:  1968-06       Impact factor: 3.857

10.  Chicken intestinal alkaline phosphatase. I. The kinetics and thermodynamics of reversible inactivation. 2. Reactivation by zinc ions.

Authors:  M KUNITZ
Journal:  J Gen Physiol       Date:  1960-07       Impact factor: 4.086
  10 in total
  2 in total

1.  The preparation of crystalline human L-lactate--nicotinamide--adenine dinucleotide oxidoreductase isoenzyme 1 involvoing preparative polyacrylamide-gel electrophoresis.

Authors:  A V Emes; N J Gallimore; A W Hodson; A L Latner
Journal:  Biochem J       Date:  1974-11       Impact factor: 3.857

2.  Light triggered detection of aminophenyl phosphate with a quantum dot based enzyme electrode.

Authors:  Waqas Khalid; Gero Göbel; Dominik Hühn; Jose-Maria Montenegro; Pilar Rivera-Gil; Fred Lisdat; Wolfgang J Parak
Journal:  J Nanobiotechnology       Date:  2011-10-07       Impact factor: 10.435
  2 in total

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