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Literature DB >> 5476728

Acid-soluble phosphorus compounds in mammalian semen.

Abstract

1. A method is described for the extraction, purification and separation of acid-soluble phosphorus compounds from mammalian semen. [8-(14)C]ATP and [8-(14)C]AMP were used as internal recovery standards to measure the breakdown and loss of these nucleotides in the procedure. 2. Bull, ram, boar and stallion semen was separated into seminal plasma and spermatozoa and the two fractions were examined separately. The overall composition of the mixture of the phosphorus compounds extracted from the two fractions was similar for the four species. 3. Glycerylphosphorylcholine and glycerylphosphorylinositol were the two phosphorus compounds identified in extracts of seminal plasma. ATP, ADP, AMP, GTP, GDP, NAD, fructose 1,6-diphosphate and glucose 6-phosphate were identified in extracts prepared from spermatozoa.

Entities: Chemical Species

Mesh：

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Year: 1970 PMID： 5476728 PMCID： PMC1179296 DOI： 10.1042/bj1180851

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

18 in total

1. Acid-soluble purine and pyrimidine compounds of mammalian spermatozoa.

Authors: K A ABRAHAM; P M BHARGAVA
Journal: Experientia Date: 1962-12-15

2. Studies on the metabolism of the protozoa. 4. Metabolism of the parasitic flagellate Strigomonas oncopelti.

Authors: J F RYLEY
Journal: Biochem J Date: 1955-03 Impact factor: 3.857

3. Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses.

Authors: R M DAWSON; N HEMINGTON; J B DAVENPORT
Journal: Biochem J Date: 1962-09 Impact factor: 3.857

4. The estimation of arginine.

Authors: H ROSENBERG; A H ENNOR; J F MORRISON
Journal: Biochem J Date: 1956-05 Impact factor: 3.857

5. Studies on the metabolism of semen: 1. General aspects. Occurrence and distribution of cytochrome, certain enzymes and coenzymes.

Authors: T Mann
Journal: Biochem J Date: 1945 Impact factor: 3.857

Acid-soluble phosphorus compounds in mammalian semen.

1. Acid-soluble purine and pyrimidine compounds of mammalian spermatozoa.

2. Studies on the metabolism of the protozoa. 4. Metabolism of the parasitic flagellate Strigomonas oncopelti.

3. Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses.

4. The estimation of arginine.

5. Studies on the metabolism of semen: 1. General aspects. Occurrence and distribution of cytochrome, certain enzymes and coenzymes.

6. An improved method for the colorimetric determination of phosphate.

7. Nucleotide metabolism. II. Chromatographic separation of acid-soluble nucleotides.

8. Analysis of phosphate esters in plant material. Extraction and purification.

9. A method for colorimetric assay of inositol and some of its phosphate derivatives.

10. The carbohydrate of human semen.

1. Effect of n-3 fatty acids and α-tocopherol on post-thaw parameters and fatty acid composition of bovine sperm.

2. Inositol lipid-mediated signalling in response to endothelin and ATP in the mammalian testis.

3. Relation between the oxidation state of nicotinamide-adenine dinucleotide and the metabolism of spermatozoa.