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Literature DB >> 5476726

Nucleotide 'maps' of digests of deoxyribonucleic acid.

Abstract

Various digests of (32)P-labelled DNA were examined by two-dimensional ionophoresis on cellulose acetate and DEAE-cellulose paper. The products from digestion with pancreatic deoxyribonuclease and Neurospora crassa endonuclease were qualitatively closely similar, but very complex, and were used to investigate the mapping behaviour of nucleotides in various ionophoretic systems. Ionophoresis on DEAE-cellulose paper in triethylamine carbonate, pH 9.7, followed by ionophoresis in the second dimension at pH1.9 gave high resolution of nucleotides in very complex mixtures and permitted the fractionation of larger quantities than is possible on cellulose acetate. High resolution of nucleotides in compact spots was obtained with two-dimensional ionophoresis on cellulose acetate and AE-cellulose paper, a system that is a useful supplement to those based on DEAE-cellulose paper.

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Year: 1970 PMID： 5476726 PMCID： PMC1179294 DOI： 10.1042/bj1180831

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

25 in total

1. THE ACTION OF STREPTONIGRIN ON BACTERIAL DNA METABOLISM AND ON INDUCTION OF PHAGE PRODUCTION IN LYSOGENIC BACTERIA.

Authors: M LEVINE; M BORTHWICK
Journal: Virology Date: 1963-12 Impact factor: 3.616

2. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.

Authors: A GAREN; C LEVINTHAL
Journal: Biochim Biophys Acta Date: 1960-03-11

3. Enzymatic synthesis of deoxyribonucleic acid. VIII. Frequencies of nearest neighbor base sequences in deoxyribonucleic acid.

Authors: J JOSSE; A D KAISER; A KORNBERG
Journal: J Biol Chem Date: 1961-03 Impact factor: 5.157

4. The frequencies of certain sequences of nucleotides in deoxyribonucleic acid.

Authors: K BURTON; G B PETERSEN
Journal: Biochem J Date: 1960-04 Impact factor: 3.857

5. The deoxyribonucleases of Escherichia coli. I. Purification and properties of a phosphodiesterase.

Authors: I R LEHMAN
Journal: J Biol Chem Date: 1960-05 Impact factor: 5.157

6. Choline phosphokinase.

Authors: J WITTENBERG; A KORNBERG
Journal: J Biol Chem Date: 1953-05 Impact factor: 5.157

7. Use of neutron activation in the characterization of small quantities of nucleic acids.

Authors: K Murray; R E Offord
Journal: Nature Date: 1966-07-23 Impact factor: 49.962

8. Structure and base sequence in the cohesive ends of bacteriophage lambda DNA.

Authors: R Wu; A D Kaiser
Journal: J Mol Biol Date: 1968-08-14 Impact factor: 5.469

9. The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5'-hydroxyl termini.

Authors: A Novogrodsky; J Hurwitz
Journal: J Biol Chem Date: 1966-06-25 Impact factor: 5.157

10. Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction.

Authors: M KUNITZ
Journal: J Gen Physiol Date: 1950-03 Impact factor: 4.086

17 in total

1. Specificity of deoxyribonucleic acid transmethylase induced by bacteriophage T2. I. Nucleotide sequences isolated from tmicrococcus luteus DNA methylated in vitro.

Authors: H van Ormondt; J Gorter; K J Havelaar; A de Waard
Journal: Nucleic Acids Res Date: 1975-08 Impact factor: 16.971

2. polA6, A mutation affecting the DNA binding capacity of DNA polymerase I.

Authors: W S Kelly; N D Grindley
Journal: Nucleic Acids Res Date: 1976-11 Impact factor: 16.971

3. A new insight into Sanger's development of sequencing: from proteins to DNA, 1943-1977.

Authors: Miguel García-Sancho
Journal: J Hist Biol Date: 2010 Impact factor: 1.326

4. The separation of oligonucleotides of baker's-yeast value transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography.

Authors: V G Gorbulev; T V Kutateladze; J Barciszewski; V D Axel'rod
Journal: Biochem J Date: 1977-06-01 Impact factor: 3.857

5. Polynucleotides associated with histone preparations.

Authors: P J Greenaway; K Murray
Journal: Biochem J Date: 1973-03 Impact factor: 3.857

6. Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

Authors: K Murray
Journal: Biochem J Date: 1973-03 Impact factor: 3.857

7. DNA nucleotide sequence restricted by the RI endonuclease.

Authors: J Hedgpeth; H M Goodman; H W Boyer
Journal: Proc Natl Acad Sci U S A Date: 1972-11 Impact factor: 11.205

8. The behaviour of oligodeoxynucleotides on thin-layer chromatography on polyethyleneimine-cellulose and ion-exchange paper electrophoresis. Applications in fractionating and sequencing terminally labelled oligodeoxynucleotides.

Authors: A Morrison; K Murray
Journal: Biochem J Date: 1974-08 Impact factor: 3.857

9. Nucleotide sequencing of DNA: preliminary characterization of the products of specific cleavages at guanine, cytosine, or adenine residues (bacteriophage M13-ribosubstitution-DNA polymerase I-electrophoresis-two-dimensional fingerprinting).

Authors: W Salser; K Fry; C Brunk; R Poon
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

10. Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.

Authors: S Bron; K Murray
Journal: Mol Gen Genet Date: 1975-12-30