Literature DB >> 5472163

The preparation of alcohol dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase from baker's yeast.

P J Butler, G M Jones.   

Abstract

A procedure has been developed for the preparation of alcohol dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase from the same sample of baker's yeast. The two enzymes were obtained in good yield in a pure crystalline form. The method minimizes the work involved in preparing the two enzymes and would be of particular advantage for preparing the enzymes in radioactive form from yeast grown in a radioactive medium.

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Year:  1970        PMID: 5472163      PMCID: PMC1179201          DOI: 10.1042/bj1180375

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  11 in total

1.  THE COMPARATIVE ENZYMOLOGY OF TRIOSEPHOSPHATE DEHYDROGENASE.

Authors:  W S ALLISON; N O KAPLAN
Journal:  J Biol Chem       Date:  1964-07       Impact factor: 5.157

2.  LOCATION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN STARCH-GELS.

Authors:  R A WILLIAMS
Journal:  Nature       Date:  1964-09-05       Impact factor: 49.962

3.  EFFECT OF COENZYMES ON HYDROGEN-DEUTERIUM EXCHANGE OF YEAST ALCOHOL DEHYDROGENASE AS DETERMINED BY INFRARED SPECTROPHOTOMETRY.

Authors:  A HVIDT; J H KAEGI
Journal:  C R Trav Lab Carlsberg       Date:  1963

4.  [Methods for the isolation of crystalline alcohol dehydrogenase].

Authors:  T KELETI
Journal:  Acta Physiol Acad Sci Hung       Date:  1958

5.  Yeast alcohol dehydrogenase: molecular weight, coenzyme binding, and reaction equilibria.

Authors:  J E HAYES; S F VELICK
Journal:  J Biol Chem       Date:  1954-03       Impact factor: 5.157

6.  Yeast glyceraldehyde-3-phosphate dehydrogenase. II. Yeast protein 2.

Authors:  E G KREBS; G W RAFTER; J M JUNGE
Journal:  J Biol Chem       Date:  1953-02       Impact factor: 5.157

7.  Yeast Glyceraldehyde-3-phosphate dehydrogenase. I. Electrophoresis of fractions precipitated by nucleic acid.

Authors:  E G KREBS
Journal:  J Biol Chem       Date:  1953-02       Impact factor: 5.157

8.  [Purity criteria for the crystallizable isoenzyme of D-glyceraldehyde-3-phosphate dehydrogenase from baker's yeast].

Authors:  K Kirschner; B Voigt
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1968-05

9.  Yeast alcohol dehydrogenase: SH groups, disulfide groups, quaternary structure, and reactivation by reductive cleavage of disulfide groups.

Authors:  M Bühner; H Sund
Journal:  Eur J Biochem       Date:  1969-11

10.  Disc electrophoresis of ribonucleic acid in polyacrylamide gels.

Authors:  E G Richards; J A Coll; W B Gratzer
Journal:  Anal Biochem       Date:  1965-09       Impact factor: 3.365

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