Determination of the equilibrium constants of associating protein systems. VI. Evaluation of M1-M wapp vs. C curves for linear and helical association.

Procedures for the determination of equilibrium constants of linear and helical associations based on molecular weight data have been derived. Theoretical construction of curves for M(1)/M(wapp) vs. C reveals two clearly identifiable patterns for linear and helical association. The change in molecular conformation between linear and helical association is evidenced by a recurring pattern of irregular curves which obviously differ from those of an indefinite stacking model.