Literature DB >> 5445613

Equilibrium density-gradient procedure for selection of synchronous cells from asynchronous cultures.

T O Sitz, A B Kent, H A Hopkins, R R Schmidt.   

Abstract

A reproducible isopycnic technique, in which linear density gradients of Ficoll are used, was developed for selecting synchronous daughter cells from asynchronous cultures of Chlorella for biochemical studies of the cell cycle.

Mesh：

Year:  1970        PMID： 5445613     DOI： 10.1126/science.168.3936.1231

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  8 in total

1.  Continuous dilution culture system for studies on gene-enzyme regulation in synchronous cultures of plant cells.

Authors:  R R Schmidt
Journal:  In Vitro       Date:  1974 Nov-Dec

2.  Purification of Synechococcus lividus by equilibrium centrifugation and its synchronization by differential centrifugation.

Authors:  T O Sitz; R R Schmidt
Journal:  J Bacteriol       Date:  1973-07       Impact factor: 3.490

3.  Isolation of plasma membranes from corn roots by sucrose density gradient centrifugation: an anomalous effect of ficoll.

Authors:  R T Leonard; W J Vanderwoude
Journal:  Plant Physiol       Date:  1976-01       Impact factor: 8.340

4.  Control of daughter-cell number variation in multiple fission: genetic versus environmental determinants in Prototheca.

Authors:  R O Poyton; D Branton
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

5.  Buoyant density variation during the cell cycle of Saccharomyces cerevisiae.

Authors:  W W Baldwin; H E Kubitschek
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

6.  Density fluctuation during the cell cycle in the defective vacuolar morphology mutants of Saccharomyces cerevisiae.

Authors:  M Ohsumi; K Uchiyama; Y Ohsumi
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

7.  Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures.

Authors:  L H Hartwell
Journal:  J Bacteriol       Date:  1970-12       Impact factor: 3.490

8.  Regulation of accumulation of ammonium-inducible glutamate dehydrogenase catalytic activity and antigen during the cell cycle of fully induced, synchronous Chlorella sorokiniana cells.

Authors:  A T Yeung; N F Bascomb; K J Turner; R R Schmidt
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490
  8 in total

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