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Literature DB >> 5441371

Mitochondrial deoxyribonucleic acid from Tetrahymena pyriformis and its kinetic complexity.

Abstract

1. Mitochondrial DNA from Tetrahymena pyriformis strain T has a buoyant density (rho) of 1.685 compared with rho1.688 for whole cell DNA. Mitochondrial preparations from T. pyriformis strain W show an enrichment of a light satellite (rho1.686), although this is not obtained free from nuclear DNA (rho1.692). 2. T. pyriformis mitochondrial DNA renatures rapidly and the kinetics of this process indicate a complexity of approx. 3x10(7) daltons. 3. The base-pairing in the renaturation product is of a precise nature, since the ;melting' temperature (80.5 degrees C) is indistinguishable from that of the native DNA (80.5 degrees C). 4. Centrifugation of mitochondrial DNA in an alkaline caesium chloride density gradient gives two bands, implying the separation of the complementary strands.

Entities: Chemical Species

Mesh：

Substances：
DNA

Year: 1970 PMID： 5441371 PMCID： PMC1185503 DOI： 10.1042/bj1160811

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

23 in total

4. Polytene chromosomes of Oxytricha: biochemical and morphological changes during macronuclear development in a ciliated protozoan.

Authors: B B Spear; M R Lauth
Journal: Chromosoma Date: 1976-01-27 Impact factor: 4.316

5. Induction of nucleolar and mitochondrial DNA replication in Tetrahymena pyriformis.

Authors: J Engberg; J R Nilsson; R E Pearlman; V Leick
Journal: Proc Natl Acad Sci U S A Date: 1974-03 Impact factor: 11.205

6. Nucleotide sequence divergence among DNA fractions of different syngens of Tetrahymena pyriformis.

Authors: S L Allen; C I Li
Journal: Biochem Genet Date: 1974-09 Impact factor: 1.890

6 in total

Mitochondrial deoxyribonucleic acid from Tetrahymena pyriformis and its kinetic complexity.

1. DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA.

2. Fractionation of nucleic acids with the methylated albumin column.

3. A fractionating column for analysis of nucleic acids.

4. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

5. Ultracentrifuge and electron microscope studies on the structure of mitochondrial DNA.

6. Heterogeneous length distribution of circular DNA filaments from yeast mitochondria.

7. The isolation of complementary strands from a mouse DNA fraction.

8. The form and size of mitochondrial DNA of the red bean, Phaseolus vulgaris.

9. Mitochondrial deoxyribonucleic acid of Tetrahymena. Its partial physical characterization.

10. Mitochondrial-satellite and circular DNA filaments in yeast.

1. Molecular hybridization of ribonucleic acid with a large excess of deoxyribonucleic acid.

2. Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii.

3. Size and configuration of Tetrahymena mitochondrial deoxyribonucleic acid.

4. Polytene chromosomes of Oxytricha: biochemical and morphological changes during macronuclear development in a ciliated protozoan.

5. Induction of nucleolar and mitochondrial DNA replication in Tetrahymena pyriformis.

6. Nucleotide sequence divergence among DNA fractions of different syngens of Tetrahymena pyriformis.