Literature DB >> 5430788

The fixation of complement and the activated first component (C1) of complement by complexes formed between antibody and divalent hapten.

N E Hyslop, R R Dourmashkin, N M Green, R R Porter.   

Abstract

Hapten-antibody complexes prepared at equivalence with the bivalent hapten bis-DNP-octamethylene-diamine and purified rabbit anti-DNP antibody were fractionated by Sepharose gel-filtration and the fractions examined by electron microscopy. Individual fractions were tested for whole-complement fixation and C1 fixation. Dimer forms did not show this type of biological activity, while fractions containing tetramers and larger polymers exhibited both C and C1 fixation, which could be inhibited by prior exposure of the complexes to the univalent hapten epsilon-DNP-caproic acid. The dose-response result indicated that the C-fixation observed was not due to interpolymeric cooperative effects. It was concluded that in the generation of biological activity by soluble antigen-antibody complexes made with complement-fixing antibody, quaternary structural changes following specific combination with antigen may be as important as any tertiary structural alterations that occur in the individual immunoglobulin molecule.

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Year:  1970        PMID： 5430788      PMCID： PMC2138777          DOI： 10.1084/jem.131.4.783

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  19 in total

1.  EXCITATION ENERGY TRANSFER AND THE QUANTITATIVE STUDY OF THE ANTIBODY HAPTEN REACTION.

Authors:  S F Velick; C W Parker; H N Eisen
Journal:  Proc Natl Acad Sci U S A       Date:  1960-11       Impact factor: 11.205

2.  Electron microscopy of an antibody-hapten complex.

Authors:  R C Valentine; N M Green
Journal:  J Mol Biol       Date:  1967-08-14       Impact factor: 5.469

Review 3.  Electron microscopy of the immunoglobulins.

Authors:  N M Green
Journal:  Adv Immunol       Date:  1969       Impact factor: 3.543

4.  The C'la fixation and transfer test: examples of its applicability to the detection and enumeration of antigens and antibodies at cell surfaces.

Authors:  T Borsos; H R Colten; J S Spalter; N Rogentine; H J Rapp
Journal:  J Immunol       Date:  1968-09       Impact factor: 5.422

5.  Characterization of rabbit fast and slow 6-6S IgG.

Authors:  W P Faulk; K W Pondman
Journal:  Immunochemistry       Date:  1969-03

6.  Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli.

Authors:  R C Valentine; B M Shapiro; E R Stadtman
Journal:  Biochemistry       Date:  1968-06       Impact factor: 3.162

7.  Titration of the first component of complement on a molecular basis: suitability of IgM and unsuitability of IgG hemolysins as sensitizer.

Authors:  H R Colten; T Borsos; H J Rapp
Journal:  Immunochemistry       Date:  1969-05

8.  Hemolysin titration based on fixation of the activated first component of complement: evidence that one molecule of hemolysin suffices to sensitize an erythrocyte.

Authors:  T Borsos; H J Rapp
Journal:  J Immunol       Date:  1965-09       Impact factor: 5.422

9.  Heterogeneity in the complementation of polypeptide subunits of a purified antibody isolated from an individual rabbit.

Authors:  R Hong; A Nisonoff
Journal:  J Immunol       Date:  1966-04       Impact factor: 5.422

10.  Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum.

Authors:  R A Nelson; J Jensen; I Gigli; N Tamura
Journal:  Immunochemistry       Date:  1966-03
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  23 in total

1.  Activation of antibody Fc function by antigen-induced conformational changes.

Authors:  J C Brown; M E Koshland
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

2.  Soluble oligovalent antigen--antibody complexes. II. The effect of various selective forces upon relative stability of isolated complexes.

Authors:  D W Knutson; L A van Es; B S Kayser; R J Glassock
Journal:  Immunology       Date:  1979-06       Impact factor: 7.397

3.  Effector activating determinants on IgG. I. The distribution and factors influencing the display of complement, neutrophil and cytotoxic B-cell determinants on human IgG sub-classes.

Authors:  I C MacLennan; A Howard; F M Gotch; P G Quie
Journal:  Immunology       Date:  1973-09       Impact factor: 7.397

4.  Circulating immune complexes in inflammatory bowel disease.

Authors:  D P Jewell; I C MacLennan
Journal:  Clin Exp Immunol       Date:  1973-06       Impact factor: 4.330

5.  Immune complexes: biochemical and biological aspects.

Authors:  P Seth
Journal:  Indian J Pediatr       Date:  1983 Sep-Oct       Impact factor: 1.967

6.  Classes and subclasses of rat antibodies: reaction with the antigen and interaction of the complex with the complement system.

Authors:  G A Medgyesi; K Miklós; J Kulics; G Füst; J Gergely; H Bazin
Journal:  Immunology       Date:  1981-05       Impact factor: 7.397

7.  The importance of quaternary structure in the expression of the C1-binding site of IgM.

Authors:  M O Bubb; J D Conradie
Journal:  Immunology       Date:  1976-12       Impact factor: 7.397

8.  Influence of aggregate size on the binding and activation of the first component of human complement by soluble IgG aggregates.

Authors:  G Doekes; L A Vanes; M R Daha
Journal:  Immunology       Date:  1982-04       Impact factor: 7.397

9.  C1- inactivator: its efficiency as a regulator of classical complement pathway activation by soluble IgG aggregates.

Authors:  G Doekes; L A van Es; M R Daha
Journal:  Immunology       Date:  1983-06       Impact factor: 7.397

10.  Membrane-controlled depletion of complement activity by spin-label-specific IgM.

Authors:  G M Humphries; H M McConnell
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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