Literature DB >> 5418476

Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety.

E H Kolodny, R O Brady, J M Quirk, J N Kanfer.   

Abstract

A procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-(3)H-D-mannosamine. Radioactive gangliosides were isolated and selectively degraded with bacterial neuraminidase and rat liver beta-galactosidase to Tay-Sachs ganglioside-(3)H. Radioactivity in the labeled product was confined to the N-acetyl-neuraminic acid portion of the molecule.

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Year:  1970        PMID： 5418476

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  5 in total

1.  In vivo studies on ganglioside metabolsim.

Authors:  J N Kanfer; D A Ellis
Journal:  Lipids       Date:  1971-12       Impact factor: 1.880

2.  Binding of bacterial toxins to glycoproteins in the envelopes of rainbow trout eggs.

Authors:  S Kudo; S Yazawa
Journal:  Histochem J       Date:  1995-04

3.  The metabolism of Tay-Sachs ganglioside: catabolic studies with lysosomal enzymes from normal and Tay-Sachs brain tissue.

Authors:  J F Tallman; W G Johnson; R O Brady
Journal:  J Clin Invest       Date:  1972-09       Impact factor: 14.808

4.  The biosynthesis of brain gangliosides. Separation of membranes with different ratios of ganglioside sialylating activity to gangliosides.

Authors:  C A Landa; H J Maccioni; A Arce; R Caputto
Journal:  Biochem J       Date:  1977-12-15       Impact factor: 3.857

5.  Enzymatic block in the synthesis of gangliosides in DNA virus-transformed tumorigenic mouse cell lines.

Authors:  F A Cumar; R O Brady; E H Kolodny; V W McFarland; P T Mora
Journal:  Proc Natl Acad Sci U S A       Date:  1970-10       Impact factor: 11.205
  5 in total

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