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Literature DB >> 5415231

A comparison between heterogeneous nuclear RNA and polysomal messenger RNA in HeLa cells by RNA-DNA hybridization.

Abstract

Heterogeneous nuclear RNA (HnRNA) and mRNA from cytoplasmic polyribosomes of HeLa cells have been compared by RNA-DNA hybridization tests. 1 microg of HeLa cell DNA binds 0.05-0.10 microg of either HnRNA or mRNA. In addition, HeLa DNA that is preexposed to unlabeled HnRNA was found to have a reduced capacity to bind either HnRNA or mRNA. The results are compatible with considerable sequence similarity in the two types of RNA but, as is discussed, firm conclusions are precluded by imperfections of the hybridization reaction as presently employed.

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Year: 1970 PMID： 5415231 PMCID： PMC2107980 DOI： 10.1083/jcb.44.3.467

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

24 in total

1. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS.

Authors: R K RALPH; A R BELLAMY
Journal: Biochim Biophys Acta Date: 1964-05-18

2. ENTRANCE OF NEWLY FORMED MESSENGER RNA AND RIBOSOMES INTO HELA CELL CYTOPLASM.

Authors: M GIRARD; H LATHAM; S PENMAN; J E DARNELL
Journal: J Mol Biol Date: 1965-02 Impact factor: 5.469

3. Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells.

Authors: K SCHERRER; H LATHAM; J E DARNELL
Journal: Proc Natl Acad Sci U S A Date: 1963-02-15 Impact factor: 11.205

4. Genetic regulatory mechanisms in the synthesis of proteins.

Authors: F JACOB; J MONOD
Journal: J Mol Biol Date: 1961-06 Impact factor: 5.469

5. Related base sequences in the DNA of simple and complex organisms. I. DNA-DNA duplex formation and the incidence of partially related base sequences in DNA.

Authors: B J McCarthy; B L McConaughy
Journal: Biochem Genet Date: 1968-06 Impact factor: 1.890

6. Molecular weights of some HeLa ribosomal RNA's.

Authors: E H McConkey; J W Hopkins
Journal: J Mol Biol Date: 1969-02-14 Impact factor: 5.469

7. Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system.

Authors: G Attardi; P C Huang; S Kabat
Journal: Proc Natl Acad Sci U S A Date: 1965-07 Impact factor: 11.205

8. Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.

Authors: G Attardi; H Parnas; M I Hwang; B Attardi
Journal: J Mol Biol Date: 1966-09 Impact factor: 5.469

9. Distribution of mRNA in the cytoplasmic polyribosomes of the HeLa cell.

Authors: H Latham; J E Darnell
Journal: J Mol Biol Date: 1965-11 Impact factor: 5.469

10. Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus).

Authors: W G Flamm; P M Walker; M McCallum
Journal: J Mol Biol Date: 1969-03-28 Impact factor: 5.469

11 in total

1. E.B. Wilson Lecture, 1998. Eukaryotic RNAs: once more from the beginning.

Authors: J E Darnell
Journal: Mol Biol Cell Date: 1999-06 Impact factor: 4.138

Review 2. [Structure and function of chromatin (author's transl)].

Authors: E Harbers; W Sandritter
Journal: Klin Wochenschr Date: 1973-07-01

3. Double-stranded regions in heterogeneous nuclear RNA from Hela cells.

Authors: W Jelinek; J E Darnell
Journal: Proc Natl Acad Sci U S A Date: 1972-09 Impact factor: 11.205

4. Adenovirus messenger RNA in mammalian cells: failure of polyribosome association in the absence of nuclear cleavage.

Authors: P M McGuire; C Swart; L D Hodge
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

5. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.

Authors: L Philipson; R Wall; G Glickman; J E Darnell
Journal: Proc Natl Acad Sci U S A Date: 1971-11 Impact factor: 11.205

6. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.

Authors: J E Darnell; R Wall; R J Tushinski
Journal: Proc Natl Acad Sci U S A Date: 1971-06 Impact factor: 11.205