| Literature DB >> 5409641 |
Abstract
An absorption peak for the peptide bond at 187 nanometers has been confirmed; a protein assay at this wavelength allows quantitation of proteins in aqueous solution at concentrations between 0.1 and 25 micrograms per milliliter. Assays are conducted at 6 degrees C to take advantage of the reduction of end-absorption of water with temperature.Entities:
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Year: 1970 PMID: 5409641 DOI: 10.1126/science.167.3915.179
Source DB: PubMed Journal: Science ISSN: 0036-8075 Impact factor: 47.728