Literature DB >> 5365346

Use of activated charcoal in the radioimmunoassay of human growth hormone in plasma.

H S Jacobs.   

Abstract

The use of activated charcoal in the radioimmunoassay of human growth hormone is described. The method permits the rapid separation of bound from free labelled human growth hormone in large batches and affords a quick method for screening iodination eluates. The necessity for an equality of protein concentration for the incubation and the separation procedure is emphasized. The method is simple, reliable and rapid, and the sensitivity obtained compares favourably with that of other methods of separation.

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Year:  1969        PMID： 5365346      PMCID： PMC474366          DOI： 10.1136/jcp.22.6.710

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  11 in total

1.  ASSAY OF HUMAN GROWTH HORMONE IN PREGNANCY AT PARTURITION AND IN LACTATION. DETECTION OF A GROWTH-HORMONE-LIKE SUBSTANCE FROM THE PLACENTA.

Authors:  F C GREENWOOD; W M HUNTER; A KLOPPER
Journal:  Br Med J       Date:  1964-01-04

2.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

3.  Iodoinsulin used to determine specific activity of iodine-131.

Authors:  S A Berson; R S Yalow
Journal:  Science       Date:  1966-04-08       Impact factor: 47.728

4.  Application of the coated charcoal separation method to the radio-immunoassay of plasma corticotrophin.

Authors:  R A Donald
Journal:  J Endocrinol       Date:  1968-08       Impact factor: 4.286

5.  Separation of antibody-bound and unbound peptide hormones labelled with iodine-131 by talcum powder and precipitated silica.

Authors:  G Rosselin; R Assan; R S Yalow; S A Berson
Journal:  Nature       Date:  1966-10-22       Impact factor: 49.962

6.  A sensitive double antibody radioimmunoassay for human growth hormone (HGH): levels of serum HGH following rapid tolbutamide infusion.

Authors:  G Boden; J S Soeldner
Journal:  Diabetologia       Date:  1967-10       Impact factor: 10.122

7.  Preliminary report on coated charcoal immunoassay of human chorionic "growth hormone-prolactin" and growth hormone.

Authors:  K S Lau; C W Gottlieb; V Herbert
Journal:  Proc Soc Exp Biol Med       Date:  1966-10

8.  A rapid method for checking the purity of radioiodinated human luteinizing hormone.

Authors:  G E Abraham
Journal:  Clin Chem       Date:  1969-03       Impact factor: 8.327

9.  Radioimmunoassay for human follicle-stimulating hormone: physiological studies.

Authors:  W D Odell; A F Parlow; C M Cargille; G T Ross
Journal:  J Clin Invest       Date:  1968-12       Impact factor: 14.808

10.  RAPID CHARCOAL ASSAY FOR INTRINSIC FACTOR (IF), GASTRIC JUICE UNSATURATED B12 BINDING CAPACITY, ANTIBODY TO IF, AND SERUM UNSATURATED B12 BINDING CAPACITY.

Authors:  K S GOTTLIEBLAU; L R WASSERMAN; V HERBERT
Journal:  Blood       Date:  1965-06       Impact factor: 22.113
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  8 in total

1.  Management of acromegaly.

Authors:  J D Nabarro
Journal:  J Clin Pathol Suppl (Assoc Clin Pathol)       Date:  1976

2.  Radioimmunoassay of antidiuretic hormone during morphine anaesthesia.

Authors:  D M Philbin; N E Wilson; J Sokoloski; C Coggins
Journal:  Can Anaesth Soc J       Date:  1976-05

3.  A new rapid gastrin radioimmunoassay.

Authors:  S Meryn; E Straus
Journal:  Dig Dis Sci       Date:  1986-06       Impact factor: 3.199

4.  Diurnal variation on oral glucose tolerance: insulin and growth hormone changes with special reference to women taking oral contraceptives.

Authors:  N W Oakley; D Monier; V Wynn
Journal:  Diabetologia       Date:  1973-06       Impact factor: 10.122

5.  The relation of growth hormone to altered carbohydrate metabolism in women taking oral contraceptives.

Authors:  D S Maw; V Wynn
Journal:  J Clin Pathol       Date:  1972-04       Impact factor: 3.411

6.  Plasma growth hormone in the New Zealand obese mouse.

Authors:  R G Larkins
Journal:  Diabetologia       Date:  1971-10       Impact factor: 10.122

7.  Radioimmunoassay of human growth hormone: technique and application to plasma, cerebrospinal fluid, and pituitary extracts.

Authors:  F J Thomas; H M Lloyd; M J Thomas
Journal:  J Clin Pathol       Date:  1972-09       Impact factor: 3.411

8.  Response of plasma pancreatic and gastrointestinal hormones and growth hormone to oral and intravenous glucose and insulin hypoglycaemia in Chagas's disease.

Authors:  R G Long; R H Albuquerque; A Prata; A J Barnes; T E Adrian; N D Christofides; S R Bloom
Journal:  Gut       Date:  1980-09       Impact factor: 23.059
  8 in total

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