Molecular distribution within collagen gel columns.

Purified collagen columns with collagen concentrations of 5 and 10% were constructed by modification of the procedure for the 1% gel as described by Shaw and Schy. The resulting columns were calibrated by elution of various tracers, and the observations compared with those of the 1% collagen gel column. The data were fitted to the Ogston gel model as formulated by Laurent and Killander, but the model was found to be not fully applicable. The thermodynamic treatment of Hjertén was applied to the data with more satisfactory agreement.