| Literature DB >> 5361212 |
J M Lebeault, B Roche, Z Duvnjak, E Azoulay.
Abstract
A method for the preparation of protoplasts from Candida tropicalis cultivated on n-tetradecane is described. This essentially consists of replacing the mannitol-sorbitol solution of the classical helicase technique by 1 m magnesium sulfate and lowering the pH to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming oxygen in the presence of n-tetradecane, n-decane, 1-decanol, and glucose.Entities:
Mesh:
Substances:
Year: 1969 PMID: 5361212 PMCID: PMC250297 DOI: 10.1128/jb.100.3.1218-1221.1969
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490