Literature DB >> 535905

Improved screening test for abnormal hemoglobins from dried blood samples.

K Altland, M Kaempfer, H Granda.   

Abstract

A method is described wherein blood samples taken from adults or newborns and dried on filter paper can be used for hemoglobin analysis within 2 years after sampling. The samples are eluted in 8 M urea in the presence of 5% 2-mercaptoethanol and 2% of the neutral detergent Nonidet P-40. Then the individual alpha, beta, gamma, and epsilon chains are separated by means of electrofocusing in 8 M urea-PAA gels. Up to 96 samples can be applied to a gel using multiple syringes. Several hundred samples can be analyzed daily by one person. This method may be especially useful for preventive programs against sickle cell anemia as well as for human mutation monitoring systems.

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Year:  1979        PMID： 535905     DOI： 10.1007/BF00289457

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  4 in total

1.  A new form of the solubility test for hemoglobin S; results from a survey of 3000 cases.

Authors:  L Heredero; H Granda; A Dorticós
Journal:  Clin Chim Acta       Date:  1976-09-20       Impact factor: 3.786

2.  An economic high speed electrophoretic screening system for hemoglobin S and other proteins.

Authors:  L Heredero; H Granda; J A Aguiar; K Altland
Journal:  Humangenetik       Date:  1974-02-21

3.  Detection of the sickle gene in the human fetus. Potential for intrauterine diagnosis of sickle-cell anemia.

Authors:  Y W Kan; A M Dozy; B P Alter; F D Frigoletto; D G Nathan
Journal:  N Engl J Med       Date:  1972-07-06       Impact factor: 91.245

4.  Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).

Authors:  J B Clegg; M A Naughton; D J Weatherball
Journal:  J Mol Biol       Date:  1966-08       Impact factor: 5.469
  4 in total
  2 in total

1.  Fetal hemoglobin variants in 80,000 Japanese neonates: high prevalence of Hb F Yamaguchi (A gamma T 80 Asp----Asn).

Authors:  Y Wada; T Fujita; K Kidoguchi; A Hayashi
Journal:  Hum Genet       Date:  1986-03       Impact factor: 4.132

2.  Double one-dimensional electrophoresis of human serum transferrin: a new high-resolution screening method for genetically determined variation.

Authors:  K Altland; R Hackler; W Knoche
Journal:  Hum Genet       Date:  1980       Impact factor: 4.132
  2 in total

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