Literature DB >> 534526

Isolation of material displaying insulin-like immunological biological activity from the brain of the blowfly Calliphora vomitoria.

H Duve, A Thorpe, N R Lazarus.   

Abstract

An insulin-like material from the brain of the blowfly Calliphora vomitoria was partially purified by acid alcohol extraction, gel filtration and ion-exchange cellulose chromatography. In addition, the RF value on polyacrylamide-gel electrophoresis was determined. The material was characterized by its ability to cross-react with bovine insulin antibody and by displaying diminished immunoreactivity on dilution. It displaced specifically bound 125I-labelled insulin from rat liver plasma membrane insulin receptors and displayed insulin-like biological activity on the isolated rat fat-cell. Within 30 min of injection into Calliphora, made hypertrehalocaemic and hyperglucaemic as a result of median neurosecretory cell removal, it caused the concentrations of both sugars to return to normal. The hypothesis is put forward that the median neurosecretory cells are the source of the material.

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Year:  1979        PMID： 534526      PMCID： PMC1161755          DOI： 10.1042/bj1840221

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  The presence of insulin in and some effects of exogenous insulin on hymenoptera tissues and body fluids.

Authors:  J Ishay; S Gitter; R Galun; M Doron; Z Laron
Journal:  Comp Biochem Physiol A Comp Physiol       Date:  1976

2.  INSULIN-LIKE ACTIVITY IN LARVAL FOODS OF THE HONEYBEE.

Authors:  P K DIXIT; N G PATEL
Journal:  Nature       Date:  1964-04-11       Impact factor: 49.962

3.  A protein extract from Drosophila melanogaster with insulin-like activity.

Authors:  P Meneses; M De Los Angeles Ortíz
Journal:  Comp Biochem Physiol A Comp Physiol       Date:  1975-06-01

4.  A simple free fat cell bioassay for insulin.

Authors:  A J Moody; M A Stan; M Stan; J Gliemann
Journal:  Horm Metab Res       Date:  1974-01       Impact factor: 2.936

5.  Control of the medial neurosecretory cells by the corpus allatum in Calliphora erythrocephala.

Authors:  E Thomsen; A O Lea
Journal:  Gen Comp Endocrinol       Date:  1969-02       Impact factor: 2.822

6.  The presence of a hypoglucemic and hypotrehalocemic hormone in the neurosecretory system of the blowfly Calliphora erythrocephala.

Authors:  H Duve
Journal:  Gen Comp Endocrinol       Date:  1978-09       Impact factor: 2.822

7.  Insulin-like hypoglycemic and immunological activities in honeybee royal jelly.

Authors:  K J Kramer; H S Tager; C N Childs; R D Speirs
Journal:  J Insect Physiol       Date:  1977       Impact factor: 2.354

8.  Hormonal regulation of trehalose metabolism in the blowfly, Phormia regina: interaction between hypertrehalosemic and hypotrehalosemic hormones.

Authors:  A C Chen; S Friedman
Journal:  J Insect Physiol       Date:  1977       Impact factor: 2.354

9.  The control of insulin receptors in the New Zealand obese mouse.

Authors:  D Baxter; N R Lazarus
Journal:  Diabetologia       Date:  1975-08       Impact factor: 10.122

10.  Mapping of the residues responsible for the negative cooperativity of the receptor-binding region of insulin.

Authors:  P De Meyts; E Van Obberghen; J Roth
Journal:  Nature       Date:  1978-06-15       Impact factor: 49.962
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  23 in total

Review 1.  Neuropeptides--occurrence and functions in insects.

Authors:  H Penzlin
Journal:  Naturwissenschaften       Date:  1989-06

Review 2.  Development of hormone receptors: conclusion.

Authors:  K D Döhler
Journal:  Experientia       Date:  1986-07-15

Review 3.  Receptors for intercellular messenger molecules in microbes: similarities to vertebrate receptors and possible implications for diseases in man.

Authors:  D LeRoith; C Roberts; M A Lesniak; J Roth
Journal:  Experientia       Date:  1986-07-15

4.  Isolation of an insulin-like peptide from the Asian malaria mosquito, Anopheles stephensi, that acts as a steroidogenic gonadotropin across diverse mosquito taxa.

Authors:  Andrew B Nuss; Mark R Brown
Journal:  Gen Comp Endocrinol       Date:  2017-05-11       Impact factor: 2.822

5.  Immunocytochemical demonstration of peptidergic neurons in the central nervous system of the pond snail Lymnaea stagnalis with antisera raised to biologically active peptides of vertebrates.

Authors:  L P Schot; H H Boer; D F Swaab; S Van Noorden
Journal:  Cell Tissue Res       Date:  1981       Impact factor: 5.249

6.  Immunocytochemical localization of peptidergic neurons and neurosecretory cells in the neuro-endocrine system of the Colorado potato beetle with antisera to vertebrate regulatory peptides.

Authors:  J A Veenstra; H M Romberg-Privee; H Schooneveld; J M Polak
Journal:  Histochemistry       Date:  1985

7.  Localization of insulin-like immunoreactivity in the synganglion of nymphal and adult Dermacentor variabilis (Acari: Ixodidae).

Authors:  H H Davis; E M Dotson; J H Oliver
Journal:  Exp Appl Acarol       Date:  1994-02       Impact factor: 2.132

8.  Localisation of pancreatic polypeptide (PP)-like immunoreactive material in neurones of the brain of the blowfly, Calliphora erythrocephala (Diptera).

Authors:  H Duve; A Thorpe
Journal:  Cell Tissue Res       Date:  1980       Impact factor: 5.249

9.  Immunocytochemical localization of gastrin-releasing peptide/bombesin-like immunoreactive neurons in insects.

Authors:  J A Veenstra; N Yanaihara
Journal:  Histochemistry       Date:  1984

10.  A neuropeptide of the blowfly Calliphora vomitoria with an amino acid composition homologous with vertebrae pancreatic polypeptide.

Authors:  H Duve; A Thorpe; N R Lazarus; P J Lowry
Journal:  Biochem J       Date:  1982-02-01       Impact factor: 3.857
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