Literature DB >> 5331371

Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis.

E Z Ron, R E Kohler, B D Davis.   

Abstract

Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.

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Year:  1966        PMID： 5331371     DOI： 10.1126/science.153.3740.1119

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  66 in total

1.  The heat shock protein YbeY is required for optimal activity of the 30S ribosomal subunit.

Authors:  Aviram Rasouly; Chen Davidovich; Eliora Z Ron
Journal:  J Bacteriol       Date:  2010-07-16       Impact factor: 3.490

2.  HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions.

Authors:  Yanqing Zhang; Chandra Sekhar Mandava; Wei Cao; Xiaojing Li; Dejiu Zhang; Ningning Li; Yixiao Zhang; Xiaoxiao Zhang; Yan Qin; Kaixia Mi; Jianlin Lei; Suparna Sanyal; Ning Gao
Journal:  Nat Struct Mol Biol       Date:  2015-10-12       Impact factor: 15.369

3.  The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits.

Authors:  J Mattias Lövgren; Göran O Bylund; Manoj K Srivastava; L A Carina Lundberg; Olof P Persson; Gunnar Wingsle; P Mikael Wikström
Journal:  RNA       Date:  2004-11       Impact factor: 4.942

4.  Deleterious mutations in small subunit ribosomal RNA identify functional sites and potential targets for antibiotics.

Authors:  Aymen Yassin; Kurt Fredrick; Alexander S Mankin
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-03       Impact factor: 11.205

5.  Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples.

Authors:  A K Bej; M H Mahbubani; J L Dicesare; R M Atlas
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

6.  YbeY, a heat shock protein involved in translation in Escherichia coli.

Authors:  Aviram Rasouly; Miriam Schonbrun; Yotam Shenhar; Eliora Z Ron
Journal:  J Bacteriol       Date:  2009-01-30       Impact factor: 3.490

7.  Dominant lethal mutations in a conserved loop in 16S rRNA.

Authors:  T Powers; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

8.  Formation of N-ethylmaleimide (NEM)-glutathione conjugate and N-ethylmaleamic acid revealed by mass spectral characterization of intracellular and extracellular microbial metabolites of NEM.

Authors:  Elmer-Rico E Mojica; Sungpyo Kim; Diana S Aga
Journal:  Appl Environ Microbiol       Date:  2007-11-02       Impact factor: 4.792

9.  Nutrition of Myxococcus xanthus FBa and some of its auxotrophic mutants.

Authors:  H E Hemphill; S A Zahler
Journal:  J Bacteriol       Date:  1968-03       Impact factor: 3.490

10.  Changes in ribosomal activity of Escherichia coli cells during prolonged culture in sea salts medium.

Authors:  D L Kalpaxis; P Karahalios; M Papapetropoulou
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490
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