| Literature DB >> 5327904 |
Abstract
Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), and E. C. C. Lin. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J. Bacteriol. 91:1763-1766. 1966.-A glycerol kinase mutant site has been mapped by transduction and sexual conjugation. Three-factor crosses with the two procedures yielded the following gene order: arginine-1-methionine-1-glycerol kinase-isoleucine, valine-16. An additional 13 independent glycerol kinase mutant sites mapped in the same region. Since some of the mutants were able to produce a protein serologically indistinguishable from the wild-type enzyme, it is concluded that the region mapped represents the structural gene for the kinase.Entities:
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Year: 1966 PMID: 5327904 PMCID: PMC316119 DOI: 10.1128/jb.91.5.1763-1766.1966
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490