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Literature DB >> 5289363

The detection of ionophorous antibiotic-cation complexes in water with fluorescent probes.

Abstract

The binding of alkali cations by the ionophorous antibiotics valinomycin, nigericin, alamethicin, and the macrotetralide actins has been shown to occur, in aqueous media, by the use of the fluorescent probes 1-anilino-8-naphthalene sulfonate and 2-p-toluidinyl-6-naphthalene sulfonate. The interaction of the ionophore-cation complexes with the fluorescent dyes produced enhanced fluorescence emission, increased lifetime and polarization, and a significant blue-shift of the emission maxima of the fluorescence spectrum. At constant antibiotic and fluorophore concentrations in water, the intensity of the fluorescence emission was found to be a function of the cation concentration. This permitted relative cation affinities to be determined for alamethicin (Na(+) congruent with K(+)), valinomycin (Rb(+) > K(+) > Cs(+)), nigericin (K(+) > Rb(+) > Na(+) > Cs(+)) and trinactin (NH(4) (+) > K(+) > Rb(+) > Cs(+)).

Entities: Chemical

Mesh：

Substances：

Year: 1971 PMID： 5289363 PMCID： PMC389346 DOI： 10.1073/pnas.68.9.2037

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

22 in total

1. Antibiotic-mediated transport of alkali ions across lipid barriers.

Authors: B C Pressman; E J Harris; W S Jagger; J H Johnson
Journal: Proc Natl Acad Sci U S A Date: 1967-11 Impact factor: 11.205

2. Sarcoplasmic reticulum. 8. Use of 8-anilino-1-naphthalene sulfonate as conformational probe on biological membranes.

Authors: J Vanderkooi; A Martonosi
Journal: Arch Biochem Biophys Date: 1969-08 Impact factor: 4.013

3. Quantitative estimation of protein binding site polarity. Fluorescence of N-arylaminonaphthalenesulfonates.

Authors: D C Turner; L Brand
Journal: Biochemistry Date: 1968-10 Impact factor: 3.162

4. Action potentials induced in biomolecular lipid membranes.

Authors: P Mueller; D O Rudin
Journal: Nature Date: 1968-02-24 Impact factor: 49.962

5. [Complexes of enniatins and other antibiotics with alkali metal ions].

Authors: H K Wipf; L A Pioda; Z Stefanac; W Simon
Journal: Helv Chim Acta Date: 1968-03-11 Impact factor: 2.164

6. Proton magnetic resonance studies of the cation-binding properties of nonactin. II. Comparison of the sodium ion, potassium ion, and cesium ion complexes.

Authors: J H Prestegard; S I Chan
Journal: J Am Chem Soc Date: 1970-07-15 Impact factor: 15.419

7. [Alkali-cation specificity and carrier qualities of the antibiotics nigericin and monensin].

Authors: W K Lutz; H K Wipf; W Simon
Journal: Helv Chim Acta Date: 1970 Impact factor: 2.164

8. Structure of the K+ complex with nonactin, a macrotetrolide antibiotic possessing highly specific K+ transport properties.

Authors: B T Kilbourn; J D Dunitz; L A Pioda; W Simon
Journal: J Mol Biol Date: 1967-12-28 Impact factor: 5.469

9. Development of K+-Na+ discrimination in experimental bimolecular lipid membranes by macrocyclic antibiotics.

Authors: P Mueller; D O Rudin
Journal: Biochem Biophys Res Commun Date: 1967-02-21 Impact factor: 3.575

10. The physicochemical basis of the functioning of biological membranes: conformational specificity of the interaction of cyclodepsipeptides with membranes and of their complexation with alkali metal ions.

Authors: M M Shemyakin; Y A Ovchinnikov; V T Ivanov; V K Antonov; A M Shkrob; I I Mikhaleva; A V Evstratov; G G Malenkov
Journal: Biochem Biophys Res Commun Date: 1967-12-29 Impact factor: 3.575

11 in total

1. Resolution of cytochrome oxidase into two component complexes.

Authors: M Fry; H Vande Zande; D E Green
Journal: Proc Natl Acad Sci U S A Date: 1978-12 Impact factor: 11.205

2. Paired moving charge model of energy coupling. III. Intrinsic ionophores in energy coupling systems.

Authors: D E Green; G Blondin; R Kessler; J H Southard
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

3. Effects of unstirred layers on the steady-state zero-current conductance of bilayer membranes mediated by neutral carriers of ions.

Authors: S Ciani; F Gambale; A Gliozzi; R Rolandi
Journal: J Membr Biol Date: 1975-10-16 Impact factor: 1.843

The detection of ionophorous antibiotic-cation complexes in water with fluorescent probes.

1. Antibiotic-mediated transport of alkali ions across lipid barriers.

2. Sarcoplasmic reticulum. 8. Use of 8-anilino-1-naphthalene sulfonate as conformational probe on biological membranes.

3. Quantitative estimation of protein binding site polarity. Fluorescence of N-arylaminonaphthalenesulfonates.

4. Action potentials induced in biomolecular lipid membranes.

5. [Complexes of enniatins and other antibiotics with alkali metal ions].

6. Proton magnetic resonance studies of the cation-binding properties of nonactin. II. Comparison of the sodium ion, potassium ion, and cesium ion complexes.

7. [Alkali-cation specificity and carrier qualities of the antibiotics nigericin and monensin].

8. Structure of the K+ complex with nonactin, a macrotetrolide antibiotic possessing highly specific K+ transport properties.

9. Development of K+-Na+ discrimination in experimental bimolecular lipid membranes by macrocyclic antibiotics.

10. The physicochemical basis of the functioning of biological membranes: conformational specificity of the interaction of cyclodepsipeptides with membranes and of their complexation with alkali metal ions.

1. Resolution of cytochrome oxidase into two component complexes.

2. Paired moving charge model of energy coupling. III. Intrinsic ionophores in energy coupling systems.

3. Effects of unstirred layers on the steady-state zero-current conductance of bilayer membranes mediated by neutral carriers of ions.

4. Isolation of an electrogenic K+/Ca2+ ionophore from an ionophoroprotein of beef heart mitochondria.

5. Resolution of complex III of the mitochondrial electron transfer chain into two component complexes.

6. Use of 1-anilino-8-naphthalene-sulfonate as a probe of gastric vesicle transport.

7. Transmembrane electrophoresis of 8-anilino-1-naphthalenesulfonate through egg lecithin liposome membranes.

8. K+/Na+ selectivity in K channels and valinomycin: over-coordination versus cavity-size constraints.

9. Coupling in cytochrome c oxidase.

Review 10. Model ion channels: gramicidin and alamethicin.