| Literature DB >> 528594 |
G J Haan, S van der Heide, B G Wolthers.
Abstract
A rapid, quantitative method is described for the analysis of fatty acids from human lipids, namely serum lipids and lipids from adipose tissue biopsies. The method includes extraction of serum lipids with chloroform--methanol, hydrolysis with tetramethylammonium hydroxide, methylation with methyl iodide and N,N-dimethylformamide and gas chromatographic analysis on a Supelcoport SP-2320 column. Fat biopsies are analysed without extraction. Optimal hydrolysis conditions have been investigated.Entities:
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Year: 1979 PMID: 528594 DOI: 10.1016/s0378-4347(00)81513-3
Source DB: PubMed Journal: J Chromatogr