Comparative DNA renaturation kinetics in amphibians.

Amphibian haploid genome sizes vary from 9 x 10(8) to 8 x 10(10) nucleotide pairs. The rate of reassociation of DNA from amphibians of different genome sizes has been employed to eliminate one of the theoretical models of chromosome structure. Scaphiopus couchi, Bufo marinus, and Rana clamitans, whose haploid genome sizes are in the ratio 2:7:10, all contain sequences of DNA represented once in the haploid genome. This indicates that their chromosomes are not composed of identical lateral strands (polynemy). The relative frequencies of repetition of DNA sequences are different for the various species of amphibians. The observed frequencies of repetitive DNA sequences in amphibians do not show the relationships expected if amphibians form a polyneme series.