Literature DB >> 5277092

Rapid and complete purification of acetylcholinesterases of electric eel and erythrocyte by affinity chromatography.

J D Berman, M Young.   

Abstract

Affinity chromatography has been used to purify acetylcholinesterase both from the electric tissue of Electrophorus electricus and from bovine erythrocyte membranes. For this purpose, several specific enzymic inhibitors of each protein were synthesized and joined covalently to an insoluble support resin. AchE is selectively retained by such inhibitor-resins when highly impure solutions are chromatographed upon them. After removal from the resin, both enzymes are electrophoretically homogeneous and they may be recovered in yields of 75% or more.

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Year:  1971        PMID： 5277092      PMCID： PMC388946          DOI： 10.1073/pnas.68.2.395

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Purification of cholinesterase from ox red cells.

Authors:  M G WARRINGA; J A COHEN
Journal:  Biochim Biophys Acta       Date:  1955-02

3.  Purification of human red cell acetylcholinesterase by electrophoresis, ultracentrifugation and gradient extraction.

Authors:  C A ZITTLE; E S DELLAMONICA; J H CUSTER; R KRIKORIAN
Journal:  Arch Biochem Biophys       Date:  1955-06       Impact factor: 4.013

4.  Acetylcholinesterase, I. Large-scale purification, homogeneity, and amino Acid analysis.

Authors:  W Leuzinger; A L Baker
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

5.  Selective enzyme purification by affinity chromatography.

Authors:  P Cuatrecasas; M Wilchek; C B Anfinsen
Journal:  Proc Natl Acad Sci U S A       Date:  1968-10       Impact factor: 11.205

6.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157

7.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

8.  Affinity labeling of the acetylcholine-receptor.

Authors:  J P Changeux; T R Podleski; L Wofsy
Journal:  Proc Natl Acad Sci U S A       Date:  1967-11       Impact factor: 11.205

9.  The preparation and properties of some tritiated diazonium salts and related compounds.

Authors:  P S Traylor; S J Singer
Journal:  Biochemistry       Date:  1967-03       Impact factor: 3.162
  9 in total
  6 in total

1.  Electrostatic steering at acetylcholine binding sites.

Authors:  Robert H Meltzer; Errol Thompson; Kizhake V Soman; Xing-Zhi Song; Jerry O Ebalunode; Theodore G Wensel; James M Briggs; Steen E Pedersen
Journal:  Biophys J       Date:  2006-06-02       Impact factor: 4.033

2.  Partial purification and some properties of a cholinesterase from bush bean (Phaseolus vulgaris L.) roots.

Authors:  D H Mansfield; G Webb; D G Clark; I E Taylor
Journal:  Biochem J       Date:  1978-12-01       Impact factor: 3.857

3.  Hydrophobic chromatography: use for purification of glycogen synthetase.

Authors:  S Shaltiel; Z Er-El
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

4.  Improved method of large scale purification of acetylcholinesterase from the electric eel (Electrophorus electricus) by affinity chromatography.

Authors:  S L Chan; E Gardner; A J Trevor
Journal:  Experientia       Date:  1973-05-15

5.  Studies on the localization of the cardiac glycoside receptor.

Authors:  T W Smith; H Wagner; J E Markis; M Young
Journal:  J Clin Invest       Date:  1972-07       Impact factor: 14.808

6.  Isolation of a tripeptide (Ala-Gly-Ser) exhibiting weak acetylthiocholine hydrolyzing activity from a high-salt soluble form of monkey diaphragm acetylcholinesterase.

Authors:  L D Jayanthi; A S Balasubramanian
Journal:  Neurochem Res       Date:  1992-04       Impact factor: 3.996
  6 in total

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