Quantitative determination of praziquantel in body fluids by gas liquid chromatography.

Praziquantel was determined in body fluids by gas liquid chromatography as follows: A known amount of an internal standard and 0.1 N sodium hydroxide solution was added to the sample to be analyzed. After extraction with methyl acetate/diisopropyl ether = 30/70, the organic extract was evaporated, the residue taken up in methylacetate and an aliquot injected for glc analysis. Separation was accomplished on a OV3 silicon oil phase, and for detection and quantitation, a thermoionic FID sensitized for nitrogen-containing compounds was used. The determination limit in serum is about 0.01 micrograms/ml. The relative standard deviation for serum concentrations of 0.1 micrograms/ml was found to be 4.5%.