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Literature DB >> 5275370

Photo-affinity labeling of specific acetylcholine-binding sites on membranes.

H Kiefer, J Lindstrom, E S Lennox, S J Singer.

Abstract

Acetylcholinesterase of intact red blood cell membranes and the acetylcholine receptor at the neuromuscular junction of whole-frog sartorius muscle have been irreversibly inactivated by photo-affinity labeling with two quaternary ammonium aryl azides. The inactivation requires that the azides, at the time of their photolytic conversion to highly reactive nitrenes, are reversibly bound to the specific acetylcholine-binding sites.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1970 PMID： 5275370 PMCID： PMC283413 DOI： 10.1073/pnas.67.4.1688

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

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Authors: L WOFSY; H METZGER; S J SINGER
Journal: Biochemistry Date: 1962-11 Impact factor: 3.162

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Authors: P FATT
Journal: J Physiol Date: 1950-10-16 Impact factor: 5.182

Review 4. Covalent labeling of active sites.

Authors: S J Singer
Journal: Adv Protein Chem Date: 1967

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Authors: G D Webb
Journal: Biochim Biophys Acta Date: 1965-05-25

6. Affinity labeling of the acetylcholine-receptor.

Authors: J P Changeux; T R Podleski; L Wofsy
Journal: Proc Natl Acad Sci U S A Date: 1967-11 Impact factor: 11.205

7. Affinity labeling of the anionic site of acetylcholinesterase.

Authors: L Wofsy; D Michaeli
Journal: Proc Natl Acad Sci U S A Date: 1967-12 Impact factor: 11.205

8. Affinity labelling of protein active sites. In: Molecular properties of drug receptors.

Authors: S J Singer
Journal: Ciba Found Symp Date: 1970

9. A novel irreversible inhibitor of acetylcholinesterase specifically directed at the anionic binding site: structure-activity relationships.

Authors: B Belleau; H Tani
Journal: Mol Pharmacol Date: 1966-09 Impact factor: 4.436

10. The role of a reactive disulphide bond in the function of the acetylcholine receptor at the frog neuromuscular junction.

Authors: D Ben-Haim; E M Landau; I Silman
Journal: J Physiol Date: 1973-10 Impact factor: 5.182

Photo-affinity labeling of specific acetylcholine-binding sites on membranes.

1. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.

2. Affinity labeling-a general method for labeling the active sites of antibody and enzyme molecules.

3. The electromotive action of acetylcholine at the motor end-plate.

Review 4. Covalent labeling of active sites.

5. Affinity of benzoquinonium and ambenonium derivatives for the acetylcholine receptor, tested on the electroplax, and for acetylcholinesterase in solution.

6. Affinity labeling of the acetylcholine-receptor.

7. Affinity labeling of the anionic site of acetylcholinesterase.

8. Affinity labelling of protein active sites. In: Molecular properties of drug receptors.

9. A novel irreversible inhibitor of acetylcholinesterase specifically directed at the anionic binding site: structure-activity relationships.

10. The reaction of acetylcholinesterase with methanesulfonyl esters of quaternary quinolinium compounds.

1. Photoaffinity labeling and quaternary structure of the acetylcholine receptor from Torpedo californica.

2. Study of frog sartorius muscle acetylcholine receptor using the irreversible inhibitor TDF.

3. Photoaffinity labeling of uncoupler binding sites on mitochondrial membrane.

Review 4. Biochemical investigations of ionic channels in excitable membranes.

5. Labelling of cholinergic receptors in subcellular fractions from rat cerebral cortex.

6. The mechanism of photoaffinity labeling.

7. An examination of the utility of photogenerated reagents by using alpha-chymotrypsin.

8. Diazoacetyl subtilisin.

9. The antibody binding site. Labelling of a specific antibody against the photo-precursor of an aryl nitrene.

10. The role of a reactive disulphide bond in the function of the acetylcholine receptor at the frog neuromuscular junction.