Literature DB >> 5274465

Surveyor substrates: energy-transfer gauges of active center topography during catalysis.

S A Latt, D S Auld, B L Valee.   

Abstract

An approach is described for the simultaneous measurement of catalysis, the distance from an active-site moiety to fluorescent substrate moieties of an enzyme substrate complex, and the microenvironment of such substrate groups. It is illustrated by means of a three-component energy transfer relay system, consisting of cobalt carboxypeptidase, its fluorescent dansylated peptide substrates, and tryptophanyl residues of the enzyme. The mode of procedure can be applied generally to yield conjoint information on functional and structural aspects of active centers of enzymes.

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Year:  1970        PMID： 5274465      PMCID： PMC283363          DOI： 10.1073/pnas.67.3.1383

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  PROCEDURES FOR THE ISOLATION OF CRYSTALLINE BOVINE PANCREATIC CARBOXYPEPTIDASE A. II. ISOLATION OF CARBOXYPEPTIDASE A-ALPHA FROM PROCARBOXYPEPTIDASE A.

Authors:  D J COX; F C BOVARD; J P BARGETZI; K A WALSH; H NEURATH
Journal:  Biochemistry       Date:  1964-01       Impact factor: 3.162

2.  Carboxypeptidase A: approaches to the chemical nature of the active center and the mechanisms of action.

Authors:  B L VALLEE; J F RIORDAN; J E COLEMAN
Journal:  Proc Natl Acad Sci U S A       Date:  1963-01-15       Impact factor: 11.205

3.  Metallocarboxypeptidases: stability constants and enzymatic characteristics.

Authors:  J E COLEMAN; B L VALLEE
Journal:  J Biol Chem       Date:  1961-08       Impact factor: 5.157

4.  The structure of carboxypeptidase A. 8. Atomic interpretation at 0.2 nm resolution, a new study of the complex of glycyl-L-tyrosine with CPA, and mechanistic deductions.

Authors:  W N Lipscomb; G N Reeke; J A Hartsuck; F A Quiocho; P H Bethge
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1970-02-12       Impact factor: 6.237

5.  Kinetics of carboxypeptidase A. I. Hydrolysis of carbobenzoxyglycyl-l-phenylalanine, benzoylglycyl-l-phenylalanine, and hippuryl-dl-beta-phenyllactic acid by metal-substituted and acetylated carboxypeptidases.

Authors:  R C Davies; J F Riordan; D S Auld; B L Vallee
Journal:  Biochemistry       Date:  1968-03       Impact factor: 3.162

6.  Intramolecular transfer of excitation from tryptophan to 1-dimethylaminonaphthalene 5-sulfonamide in a series of model compounds.

Authors:  R H Conrad; L Brand
Journal:  Biochemistry       Date:  1968-02       Impact factor: 3.162

7.  Fluorescence spectroscopy of proteins.

Authors:  L Stryer
Journal:  Science       Date:  1968-11-01       Impact factor: 47.728

8.  On the size of the active site in proteases. II. Carboxypeptidase-A.

Authors:  N Abramowitz; I Schechter; A Berger
Journal:  Biochem Biophys Res Commun       Date:  1967-12-29       Impact factor: 3.575

9.  X-ray diffraction studies of enzymes.

Authors:  D M Blow; T A Steitz
Journal:  Annu Rev Biochem       Date:  1970       Impact factor: 23.643

10.  Syncatalytic modification of a functional tyrosyl residue in aspartate aminotransferase.

Authors:  P Christen; J F Riordan
Journal:  Biochemistry       Date:  1970-07-21       Impact factor: 3.162
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  11 in total

1.  The orientational freedom of molecular probes. The orientation factor in intramolecular energy transfer.

Authors:  R E Dale; J Eisinger; W E Blumberg
Journal:  Biophys J       Date:  1979-05       Impact factor: 4.033

2.  Direct observation of enzyme substrate complexes by stopped-flow fluorescence: mathematical analyses.

Authors:  R R Lobb; D S Auld
Journal:  Experientia       Date:  1984-11-15

3.  Stopped-flow cryoenzymology: detection of catalytic intermediates not observable at ambient temperatures.

Authors:  H E Van Wart; S H Lin
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205

4.  Determination of enzyme mechanisms by radiationless energy transfer kinetics.

Authors:  R R Lobb; D S Auld
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

5.  Regulation of CNGA1 Channel Gating by Interactions with the Membrane.

Authors:  Teresa K Aman; Sharona E Gordon; William N Zagotta
Journal:  J Biol Chem       Date:  2016-03-11       Impact factor: 5.157

6.  Proximity relationships in rhodopsin.

Authors:  C W Wu; L Stryer
Journal:  Proc Natl Acad Sci U S A       Date:  1972-05       Impact factor: 11.205

7.  Fluorescent derivatives of nucleotides. Metal ion interactions and pH dependency.

Authors:  J M Vanderkooi; C J Weiss; G V Woodrow
Journal:  Biophys J       Date:  1979-02       Impact factor: 4.033

8.  Energy transfer between terbium (III) and cobalt (II) in thermolysin: a new class of metal--metal distance probes.

Authors:  W D Horrocks; B Holmquist; B L Vallee
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

9.  Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET.

Authors:  William N Zagotta; Moshe T Gordon; Eric N Senning; Mika A Munari; Sharona E Gordon
Journal:  J Gen Physiol       Date:  2016-01-11       Impact factor: 4.086

10.  Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane.

Authors:  Sharona E Gordon; Eric N Senning; Teresa K Aman; William N Zagotta
Journal:  J Gen Physiol       Date:  2016-01-11       Impact factor: 4.086
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