Literature DB >> 5271760

Nuclear magnetic resonance studies of the structure and binding sites of enzymes. X. Preparation of selectively deuterated analogs of staphylococcal nuclease.

I Putter, A Barreto, J L Markley, O Jardetzky.   

Abstract

Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The (1)H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonated sites.

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Year:  1969        PMID： 5271760      PMCID： PMC223298          DOI： 10.1073/pnas.64.4.1396

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  3 in total

1.  An improved method for the purification of staphylococcal nuclease.

Authors:  S Fuchs; P Cuatrecasas; C B Anfinsen
Journal:  J Biol Chem       Date:  1967-10-25       Impact factor: 5.157

2.  Assignment of the histidine peaks in the nuclear magnetic resonance spectrum of ribonuclease.

Authors:  D H Meadows; O Jardetzky; R M Epand; H H Ruterjans; H A Scheraga
Journal:  Proc Natl Acad Sci U S A       Date:  1968-07       Impact factor: 11.205

3.  High-resolution nuclear magnetic resonance spectra of selectively deuterated staphylococcal nuclease.

Authors:  J L Markley; I Putter; O Jardetzky
Journal:  Science       Date:  1968-09-20       Impact factor: 47.728
  3 in total
  6 in total

1.  Biomolecular NMR: Past and future.

Authors:  John L Markley; William Milo Westler
Journal:  Arch Biochem Biophys       Date:  2017-05-08       Impact factor: 4.013

2.  PQQ: biosynthetic studies in Methylobacterium AM1 and Hyphomicrobium X using specific 13C labeling and NMR.

Authors:  D R Houck; J L Hanners; C J Unkefer; M A van Kleef; J A Duine
Journal:  Antonie Van Leeuwenhoek       Date:  1989-05       Impact factor: 2.271

Review 3.  Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins.

Authors:  Raffaello Verardi; Nathaniel J Traaseth; Larry R Masterson; Vitaly V Vostrikov; Gianluigi Veglia
Journal:  Adv Exp Med Biol       Date:  2012       Impact factor: 2.622

4.  Nuclear magnetic resonance studies of the structure and binding sites of enzymes. XII. A conformational equilibrium in staphylococcal nuclease involving a histidine residue.

Authors:  J L Markley; M N Williams; O Jardetzky
Journal:  Proc Natl Acad Sci U S A       Date:  1970-03       Impact factor: 11.205

5.  A Semiautomated Assignment Protocol for Methyl Group Side Chains in Large Proteins.

Authors:  Jonggul Kim; Yingjie Wang; Geoffrey Li; Gianluigi Veglia
Journal:  Methods Enzymol       Date:  2015-09-26       Impact factor: 1.600

6.  Fluorotyrosine alkaline phosphatase from Escherichia coli: preparation, properties, and fluorine-19 nuclear magnetic resonance spectrum.

Authors:  B D Sykes; H I Weingarten; M J Schlesinger
Journal:  Proc Natl Acad Sci U S A       Date:  1974-02       Impact factor: 11.205
  6 in total

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