Literature DB >> 5263006

Promotion of the autolysis of alpha-chymotrypsin by Evans blue.

A N Glazer.   

Abstract

Evans blue has been demonstrated to promote the autolysis of alpha-chymotrypsin at low dye-to-protein ratios in alkaline solution. This effect has been attributed to the stabilization of less tightly folded conformers of the protein by the dye. The effect is specific. Of 20 other strongly acidic dyes tested, only trypan red showed activity comparable to that of Evans blue. A general discussion of the influence of ligand binding on the stability of proteins is presented.

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Year:  1969        PMID： 5263006      PMCID： PMC286152          DOI： 10.1073/pnas.64.1.235

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  6 in total

Review 1.  THE CATALYTIC ENVIRONMENT AND ITS BIOLOGICAL IMPLICATIONS.

Authors:  S GRISOLIA
Journal:  Physiol Rev       Date:  1964-10       Impact factor: 37.312

2.  The spectrophotometric determination of the operational normality of an alpha-chymotrypsin solution.

Authors:  G R SCHONBAUM; B ZERNER; M L BENDER
Journal:  J Biol Chem       Date:  1961-11       Impact factor: 5.157

3.  A method for testing the purity of commercial samples of T 1824 with observations on the amounts of colored impurities present and the errors in plasma volume estimation caused by them.

Authors:  D LEESON; E B REEVE
Journal:  J Physiol       Date:  1949-08       Impact factor: 5.182

4.  The interaction of penicillinase with penicillins. IV. Structural aspects of catalytic and non-catalytic interactions.

Authors:  N Citri; N Zyk
Journal:  Biochim Biophys Acta       Date:  1965-06-22

5.  Protein substrate conformation and proteolysis.

Authors:  G Markus
Journal:  Proc Natl Acad Sci U S A       Date:  1965-07       Impact factor: 11.205

6.  The specific binding of Biebrich Scarlet to the active site of alpha-chymotrypsin.

Authors:  A N Glazer
Journal:  J Biol Chem       Date:  1967-10-10       Impact factor: 5.157
  6 in total
  1 in total

1.  [Potassium release of the isolated rat liver after treatment with evans blue. Inhibition of the effect of phalloidin in vitro and in vivo].

Authors:  W Jahn
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1972       Impact factor: 3.000
  1 in total

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