Literature DB >> 5263003

A genetic assay for mRNA's of phage T4.

R Jayaraman, E B Goldberg.   

Abstract

A method has been developed by which many gene-specific mRNA's in T4-infected cells can be quantitatively assayed. The method involves separation of complementary strands of phage T4 DNA, hybridization of the strands with RNA, digestion of nonhybridized regions of DNA with an endonuclease specific for single-stranded DNA, and assay of protected genetic markers by transformation. It has been shown that the gene gammaIIB is transcribed early from the light strand and that the gene 21 is transcribed late from the heavy strand.

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Year:  1969        PMID： 5263003      PMCID： PMC286147          DOI： 10.1073/pnas.64.1.198

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  CHARACTERIZATION OF A DEOXYRIBONUCLEASE OF MUSTELUS CANIS LIVER.

Authors:  H ASHE; E SEAMAN; H VANVUNAKIS; L LEVINE
Journal:  Biochim Biophys Acta       Date:  1965-05-18

2.  ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE.

Authors:  S Benzer
Journal:  Proc Natl Acad Sci U S A       Date:  1961-03       Impact factor: 11.205

3.  The transcription of the SV40 genome in productively infected and transformed cells.

Authors:  G Sauer; J R Kidwai
Journal:  Proc Natl Acad Sci U S A       Date:  1968-12       Impact factor: 11.205

4.  The periodicity of RNA polymerase initiations: a new regulatory feature of transcription.

Authors:  R F Baker; C Yanofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1968-05       Impact factor: 11.205

5.  Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the poly U and poly U,G binding sites.

Authors:  A Guha; W Szybalski
Journal:  Virology       Date:  1968-04       Impact factor: 3.616

6.  Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA.

Authors:  A Bolle; R H Epstein; W Salser; E P Geiduschek
Journal:  J Mol Biol       Date:  1968-02-14       Impact factor: 5.469

7.  Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA.

Authors:  M Oishi
Journal:  Proc Natl Acad Sci U S A       Date:  1968-05       Impact factor: 11.205

8.  The molecular origin of lambda prophage mRNA.

Authors:  P D Bear; A Skalka
Journal:  Proc Natl Acad Sci U S A       Date:  1969-02       Impact factor: 11.205

9.  On the transcription of the tryptophan operon in Escherichia coli. II. Production of the specific messenger RNA.

Authors:  F Imamoto; N Morikawa; K Sato; S Mishima; T Nishimura
Journal:  J Mol Biol       Date:  1965-08       Impact factor: 5.469

10.  Orientation and control of transcription in E. coli phage lambda.

Authors:  S Kumar; K Bovre; A Guha; Z Hradecna; V R Maher; W Szybalski
Journal:  Nature       Date:  1969-03-01       Impact factor: 49.962
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  4 in total

Review 1.  Transfection of Enterobacteriaceae and its applications.

Authors:  R Benzinger
Journal:  Microbiol Rev       Date:  1978-03

2.  DNA Synthesis and Gene Expression in Bacillus subtilis Infected with Wild-Type and Hypermodification-Defective Bacteriophage SP10.

Authors:  H Witmer; M Franks
Journal:  J Virol       Date:  1982-05       Impact factor: 5.103

3.  Variable transforming activity of nonglucosylated and fragmented T4 DNA.

Authors:  L A McNicol
Journal:  J Virol       Date:  1973-08       Impact factor: 5.103

4.  Purification and in vitro transcription of a transfer RNA gene.

Authors:  V Daniel; J S Beckmann; S Sarid; J I Grimberg; M Herzberg; U Z Littauer
Journal:  Proc Natl Acad Sci U S A       Date:  1971-09       Impact factor: 11.205
  4 in total

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