Literature DB >> 5261914

Solubilization and partial characterization of a phytohemagglutinin receptor site from human erythrocytes.

S Kornfeld, R Kornfeld.   

Abstract

Trypsin treatment of human erythrocytes releases a soluble glycopeptide which binds to phytohemagglutinin and abolishes the erythroagglutinating and lymphocyte-stimulating properties of this molecule. The glycopeptide has been purified by alkaline borohydride treatment, proteolytic digestion, gel filtration, and DEAE-cellulose chromatography. The most highly purified glycopeptide has a molecular weight of about 2,000. The specificity for binding to phytohemagglutinin resides in the oligosaccharide portion of the molecule with the determinant sugar being a galactose residue which is penultimate to a N-acetylneuraminic acid in some chains and uncovered in others. The glycopeptide is about 3,000 times more potent than either N-acetylgalactosamine or galactose in inhibiting the mitogenic response of lymphocytes induced by phytohemagglutinin.

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Year:  1969        PMID： 5261914      PMCID： PMC223484          DOI： 10.1073/pnas.63.4.1439

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  The thiobarbituric acid assay of sialic acids.

Authors:  L WARREN
Journal:  J Biol Chem       Date:  1959-08       Impact factor: 5.157

Review 2.  Blood-group substances.

Authors:  W M Watkins
Journal:  Science       Date:  1966-04-08       Impact factor: 47.728

3.  Studies on the glycosidases in jack bean meal. I. Isolation and properties of alpha-mannosidase.

Authors:  Y T Li
Journal:  J Biol Chem       Date:  1967-12-10       Impact factor: 5.157

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  Studies on glycopeptides released by trypsin from intact human erythrocytes.

Authors:  R J Winzler; E D Harris; D J Pekas; C A Johnson; P Weber
Journal:  Biochemistry       Date:  1967-07       Impact factor: 3.162

6.  The effect of N acetyl C-galactosamine and other sugars on the mitogenic activity and attachment of PHA to tonsil cells.

Authors:  H Borberg; I Yesner; B Gesner; R Silber
Journal:  Blood       Date:  1968-06       Impact factor: 22.113

7.  Immunochemical studies on blood groups. XLI. Proposed structures for the carbohydrate portions of blood group A, B, H, Lewis-a, and Lewis-b substances.

Authors:  K O Lloyd; E A Kabat
Journal:  Proc Natl Acad Sci U S A       Date:  1968-12       Impact factor: 11.205

8.  A sialoglycopeptide liberated from human red cells by treatment with trypsin.

Authors:  S Ohkuma; S Ikemoto
Journal:  Nature       Date:  1966-10-08       Impact factor: 49.962

9.  Separation of lymphocyte-stimulating and agglutinating activities in phytohaemagglutinin (PHA) from Phaseolus vulgaris.

Authors:  T Weber; C T Nordman; R Gräsbeck
Journal:  Scand J Haematol       Date:  1967

10.  Phytohemagglutinin: inhibition of the agglutinating activity by N-acetyl-D-galactosamine.

Authors:  H Berberg; J Woodruff; R Hirschhorn; B Gesner; P Miescher; R Silber
Journal:  Science       Date:  1966-11-25       Impact factor: 47.728
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  13 in total

Review 1.  Protein glycosylation in cancer.

Authors:  Sean R Stowell; Tongzhong Ju; Richard D Cummings
Journal:  Annu Rev Pathol       Date:  2015       Impact factor: 23.472

2.  Lymphocyte activation. 3. Binding sites for phytomitogens on lymphocyte subpopulations.

Authors:  M F Greaves; S Bauminger; G Janossy
Journal:  Clin Exp Immunol       Date:  1972-03       Impact factor: 4.330

3.  Cell-surface mucosubstances from trypsin diaggregation of normal and virus-transformed lines of baby-hamster kidney cells.

Authors:  S M Minnikin; A Allen
Journal:  Biochem J       Date:  1973-08       Impact factor: 3.857

4.  Chemical characterization and surface orientation of the major glycoprotein of the human erythrocyte membrane.

Authors:  V T Marchesi; T W Tillack; R L Jackson; J P Segrest; R E Scott
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

5.  Kinetics of the reaction of kidney-bean leucoagglutinin with human lymphocytes.

Authors:  T Weber
Journal:  Experientia       Date:  1973

6.  The effects of foetuin on lymphocytes: lymphocyte-stimulating property.

Authors:  C C Hsu; W I Waithe; P Hathaway; K Hirschhorn
Journal:  Clin Exp Immunol       Date:  1973-11       Impact factor: 4.330

7.  The immunological and physicochemical properties of mitogenic proteins derived from Phaseolus vulgaris.

Authors:  S Yachnin; R H Svenson
Journal:  Immunology       Date:  1972-05       Impact factor: 7.397

8.  Interferon: evidence for its glycoprotein nature.

Authors:  F Dorner; M Scriba; R Weil
Journal:  Proc Natl Acad Sci U S A       Date:  1973-07       Impact factor: 11.205

9.  The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles.

Authors:  T W Tillack; R E Scott; V T Marchesi
Journal:  J Exp Med       Date:  1972-06-01       Impact factor: 14.307

10.  A study on the mechanism of intercellular adhesion. Effects of neuraminidase, calcium, and trypsin on the aggregation of suspended HeLa cells.

Authors:  J J Deman; E A Bruyneel; M M Mareel
Journal:  J Cell Biol       Date:  1974-03       Impact factor: 10.539
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