Literature DB >> 5253668

Hybridization of hamster cells with high and low folate reductase activity.

J W Littlefield.   

Abstract

These studies concern the question whether diffusible repressors control the syntheses of enzymes in mammalian cells. First, clonal sublines of baby hamster kidney (BHK 21/13) cells in culture were selected in stepwise fashion for resistance to aminopterin. These sublines survived in concentrations of aminopterin that were up to 10(4) times higher than those tolerated by wild-type cells because they contained up to 125 times as much folate reductase, probably due to overproduction of the enzyme. When five resistant sublines were hybridized to wild-type lines, 32 of 35 hybrid clones contained intermediate levels of reductase activity. This suggests that overproduction of reductase is not due to loss of a diffusible repressor.

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Year:  1969        PMID： 5253668      PMCID： PMC285959          DOI： 10.1073/pnas.62.1.88

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Relation of folic acid reductase to amethopterin resistance in cultured mammalian cells.

Authors:  M T HAKALA; S F ZAKRZEWSKI; C A NICHOL
Journal:  J Biol Chem       Date:  1961-03       Impact factor: 5.157

2.  Genetic regulatory mechanisms in the synthesis of proteins.

Authors:  F JACOB; J MONOD
Journal:  J Mol Biol       Date:  1961-06       Impact factor: 5.469

3.  RETENTION OF MULTIPLE DEVELOPMENTAL POTENTIALITIES BY CELLS OF A MOUSE TESTICULAR TERATOCARCINOMA DURING PROLONGED CULTURE in vitro AND THEIR EXTINCTION UPON HYBRIDIZATION WITH CELLS OF PERMANENT LINES.

Authors:  B W Finch; B Ephrussi
Journal:  Proc Natl Acad Sci U S A       Date:  1967-03       Impact factor: 11.205

4.  Selection of morphologically normal cell lines from polyoma-transformed BHK21/13 hamster fibroblasts.

Authors:  G Marin; J W Littlefield
Journal:  J Virol       Date:  1968-01       Impact factor: 5.103

5.  Increase of dihydrofolate reductase activity in cultured mammalian cells after exposure to methotrexate.

Authors:  B L Hillcoat; V Swett; J R Bertino
Journal:  Proc Natl Acad Sci U S A       Date:  1967-10       Impact factor: 11.205

6.  Hybridization of somatic cells derived from mouse and syrian hamster: evolution of karyotype and enzyme studies.

Authors:  B R Migeon
Journal:  Biochem Genet       Date:  1968-03       Impact factor: 1.890

7.  Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. II. The level of regulation of 3,4-dihydroxyphenylalanine oxidase.

Authors:  R Davidson; K Yamamoto
Journal:  Proc Natl Acad Sci U S A       Date:  1968-07       Impact factor: 11.205

8.  Synthesis of collagen and hyaluronic acid by fibroblast hybrids.

Authors:  H Green; B Ephrussi; M Yoshida; D Hamerman
Journal:  Proc Natl Acad Sci U S A       Date:  1966-01       Impact factor: 11.205

9.  Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system.

Authors:  G Attardi; P C Huang; S Kabat
Journal:  Proc Natl Acad Sci U S A       Date:  1965-07       Impact factor: 11.205

10.  Responses of acetylcholinesterase from Torpedo marmorata to salts and curarizing drugs.

Authors:  J P Changeux
Journal:  Mol Pharmacol       Date:  1966-09       Impact factor: 4.436
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  22 in total

1.  Phase II trial of oral piritrexim in advanced, previously treated transitional cell cancer of bladder.

Authors:  M Khorsand; J Lange; L Feun; N J Clendeninn; M Collier; G Wilding
Journal:  Invest New Drugs       Date:  1997       Impact factor: 3.850

2.  Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor.

Authors:  J W Zolg; U J Hänggi; H G Zachau
Journal:  Mol Gen Genet       Date:  1978-08-04

Review 3.  Use of somatic cell hybrids for analysis of the differentiated state.

Authors:  F M Davis; E A Adelberg
Journal:  Bacteriol Rev       Date:  1973-06

Review 4.  Utilization of somatic cell hybrids for genetic studies in man.

Authors:  K H Grzeschik
Journal:  Humangenetik       Date:  1973

5.  Some aspects of somatic cell hybridization.

Authors:  J W Littlefield; S Goldstein
Journal:  In Vitro       Date:  1970 Jul-Aug

6.  Regulation of aryl hydrocarbon hydroxylase in intraspecific hybrids of human, mouse, and hamster cells.

Authors:  F J Wiebel; H V Gelboin; H G Coon
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

Review 7.  Regulation of gene expression in somatic cell hybrids: a review.

Authors:  R L Davidson
Journal:  In Vitro       Date:  1971 May-Jun

8.  Variable content of double minute chromosomes is not correlated with degree of phenotype instability in methotrexate-resistant human cell lines.

Authors:  J Masters; B Keeley; H Gay; G Attardi
Journal:  Mol Cell Biol       Date:  1982-05       Impact factor: 4.272

9.  Regulatory mutants of dihydrofolate reductase in Escherichia coli K12.

Authors:  R Sheldon; S Brenner
Journal:  Mol Gen Genet       Date:  1976-08-10

10.  Purification and properties of dihydrofolate reductase from cultured mammalian cells.

Authors:  J Gauldie; L Marshall; B L Hillcoat
Journal:  Biochem J       Date:  1973-06       Impact factor: 3.857
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