Literature DB >> 52343

Concentration of Rous sarcoma virus from tissue culture fluids with polyethylene glycol.

D L Bronson, A Y Elliott, D Ritzi.   

Abstract

Concentration of Rous sarcoma virus from tissue culture fluids with polyethylene glycol, with and without NaCl or dextran sulfate, resulted in significant and highly variable losses caused by entrapment of virus particles in proteinaceous debris. Treatment of concentrated preparations with Pronase greatly enhanced the recovery of virions. Maximum recovery of virus particles was obtained by the addition of 8% polyethylene glycol and 0.4 M NaCl to tissue culture fluids, followed by Pronase treatment of the concentrated virus preparations.

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Year:  1975        PMID： 52343      PMCID： PMC187204          DOI： 10.1128/am.30.3.464-471.1975

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  23 in total

1.  PROTEIN PRECIPITATION BY UNCHARGED WATER-SOLUBLE POLYMERS.

Authors:  M ZEPPEZAUER; S BRISHAMMER
Journal:  Biochim Biophys Acta       Date:  1965-03-29

2.  Concentration of poliovirus by an aqueous polymer two-phase system.

Authors:  E C NORRBY; P A ALBERTSSON
Journal:  Nature       Date:  1960-12-17       Impact factor: 49.962

3.  The purification and concentration of viruses by aqueous polymerphase systems.

Authors:  L PHILIPSON; P A ALBERTSSON; G FRICK
Journal:  Virology       Date:  1960-07       Impact factor: 3.616

4.  Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation".

Authors:  J McSharry; R Benzinger
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

5.  Efficient filtration and sizing of viruses with membrane filters.

Authors:  B A Ver; J L Melnick; C Wallis
Journal:  J Virol       Date:  1968-01       Impact factor: 5.103

6.  Use of water-soluble polymers for the isolation and purification of human immunoglobulins.

Authors:  P W Chun; M Fried; E F Ellis
Journal:  Anal Biochem       Date:  1967-06       Impact factor: 3.365

7.  Precipitation of some plasma proteins by the addition of dextran or polyethylene glycol.

Authors:  P H Iverius; T C Laurent
Journal:  Biochim Biophys Acta       Date:  1967-02-21

8.  Concentration of certain myxoviruses with polyethylene glycol.

Authors:  A D Kanarek; G W Tribe
Journal:  Nature       Date:  1967-05-27       Impact factor: 49.962

9.  Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures.

Authors:  J J Syrewicz; R B Naso; C S Wang; R B Arlinghaus
Journal:  Appl Microbiol       Date:  1972-09

10.  Analysis of the ribonucleic acid of murine leukemia virus.

Authors:  J P Bader; T L Steck
Journal:  J Virol       Date:  1969-10       Impact factor: 5.103
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  5 in total

1.  Evidence for the identity of shared 5'-terminal sequences between genome RNA and subgenomic mRNA's of B77 avian sarcoma virus.

Authors:  C M Stoltzfus; L K Kuhnert
Journal:  J Virol       Date:  1979-11       Impact factor: 5.103

2.  Concentration and purification of viruses and bacteriophages with polyethylene glycol.

Authors:  B P Vajda
Journal:  Folia Microbiol (Praha)       Date:  1978       Impact factor: 2.099

3.  Rapid purification of extracellular and intracellular Moloney murine leukemia virus.

Authors:  M Aboud; M Wolfson; Y Hassan; M Huleihel
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

4.  Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.

Authors:  G D Lewis; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

5.  Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales.

Authors:  Anna-Kristin Ludwig; Kyra De Miroschedji; Thorsten R Doeppner; Verena Börger; Johannes Ruesing; Vera Rebmann; Stephan Durst; Sören Jansen; Michel Bremer; Elmar Behrmann; Bernhard B Singer; Holger Jastrow; Jan Dominik Kuhlmann; Fouzi El Magraoui; Helmut E Meyer; Dirk M Hermann; Bertram Opalka; Stefan Raunser; Matthias Epple; Peter A Horn; Bernd Giebel
Journal:  J Extracell Vesicles       Date:  2018-10-17
  5 in total

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