Literature DB >> 523318

Effects of nuclear disruption on the macromolecular composition of nucleosome subfractions.

B Levy-Wilson.   

Abstract

The macromolecular composition of nucleosomal subfractions obtained by limited action of micrococcal nuclease upon trout testis chromatin has been analyzed with the purpose of comparison with the properties of transcriptionally active nucleosomal fractions derived by similar treatment of intact nuclei. The results indicate that when the native chromatin structure of intact nuclei is disrupted prior to the nuclease action, the nucleosomal subfractions that are subsequently generated have an altered composition.

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Year:  1979        PMID: 523318      PMCID: PMC342382          DOI: 10.1093/nar/7.8.2239

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  11 in total

1.  Nucleosome structure III: the structure and transcriptional activity of the chromatin containing the ovalbumin and globin genes in chick oviduct nuclei.

Authors:  M Bellard; F Gannon; P Chambon
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

2.  Release of free F1 histone during nuclease digestion of rat liver chromatin.

Authors:  C B Chae
Journal:  Biochemistry       Date:  1974-03-12       Impact factor: 3.162

3.  Partial purification of transcriptionally active nucleosomes from trout testis cells.

Authors:  B Levy; G H Dixon
Journal:  Nucleic Acids Res       Date:  1978-11       Impact factor: 16.971

4.  Limited action of micrococcal nuclease on trout testis nuclei generates two mononucleosome subsets enriched in transcribed DNA sequences.

Authors:  B Levy-Wilson; G H Dixon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

5.  A subset of trout testis nucleosomes enriched in transcribed DNA sequences contains high mobility group proteins as major structural components.

Authors:  B W Levy; W Connor; G H Dixon
Journal:  J Biol Chem       Date:  1979-02-10       Impact factor: 5.157

6.  Fractionation of hen oviduct chromatin into transcriptionally active and inactive regions after selective micrococcal nuclease digestion.

Authors:  K S Bloom; J N Anderson
Journal:  Cell       Date:  1978-09       Impact factor: 41.582

7.  Procedures for minimizing protease activity during isolation of nuclei, chromatin, and the histones.

Authors:  P Hoffmann; R Chalkley
Journal:  Methods Cell Biol       Date:  1978       Impact factor: 1.441

8.  Transcription and chromatin structure.

Authors:  B J McCarthy; J T Nishiura; D Doenecke; D S Nasser; C B Johnson
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

9.  Altered nucleosome structure containing DNA sequences complementary to 19S and 26S ribosomal RNA in Physarum polycephalum.

Authors:  E M Johnson; V G Allfrey; E M Bradbury; H R Matthews
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

10.  Diversity of sequences of polyadenylated cytoplasmic RNA from rainbow trout (Salmo gairdnerii) testis and liver.

Authors:  B Levy; G H Dixon
Journal:  Biochemistry       Date:  1977-03-08       Impact factor: 3.162

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