| Literature DB >> 521632 |
K A Joiner, R Shahon, J A Gelfand.
Abstract
A microhemolytic assay for measurement of murine alternative complement pathway activity is described. The assay uses 51Cr release from neuraminidase-treated rabbit erythrocytes incubated with Mg2+ EGTA-chelated murine serum. Neuraminidase pretreatment of rabbit erythrocytes increases the sensitivity of the assay 8--10-fold, enable the use of small volumes of individual mouse sera. The assay affords a simple, sensitive and reproducible method for measuring murine alternative complement pathway activity. Significant differences were found between strains alternative complement pathway activity of serum from various inbred murine lines was measured.Entities:
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Year: 1979 PMID： 521632 DOI： 10.1016/0022-1759(79)90141-8
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303