Literature DB >> 52163

Immunological recognition of specific antigenic determinants in UV- and gamma-irradiated phage DNA.

G Hotz.   

Abstract

Thermally denatured DNA of coliphage T1 after treatment with uv-light (2537 A) and 60Co-gamma rays acts as a hapten with antigenic determinant groups specific for radiation-induced alterations of the macromolecule. After conjugation to methylated bovine serum albumin the DNA becomes immunogenic in rabbits. Antibodies against irradiated DNA do not react with unirradiated single-stranded DNA. Antigen-antibody complexes were demonstrated by CsCl-density gradient centrifugation. The decrease in buoyant density of the DNA is proportional to the amount of antibody protein bound to the antigen. By this means photoproducts as well as alterations due to ionizing radiation in DNA were detected independent of the type of antigen-antibody complex, i.e. precipitating or soluble aggregate.

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Year:  1975        PMID： 52163     DOI： 10.1007/bf02339808

Source DB:  PubMed          Journal:  Radiat Environ Biophys        ISSN： 0301-634X            Impact factor:   1.925


  9 in total

1.  PRODUCTION OF ANTIBODIES TO DENATURED DEOXYRIBONUCLEIC ACID (DNA).

Authors:  O J PLESCIA; W BRAUN; N C PALCZUK
Journal:  Proc Natl Acad Sci U S A       Date:  1964-08       Impact factor: 11.205

2.  RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.

Authors:  R P BOYCE; P HOWARD-FLANDERS
Journal:  Proc Natl Acad Sci U S A       Date:  1964-02       Impact factor: 11.205

3.  THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.

Authors:  R B SETLOW; W L CARRIER
Journal:  Proc Natl Acad Sci U S A       Date:  1964-02       Impact factor: 11.205

4.  Fractionation of nucleic acids with the methylated albumin column.

Authors:  N SUEOKA; T Y CHENG
Journal:  J Mol Biol       Date:  1962-03       Impact factor: 5.469

5.  Infectious DNA from coliphage T1. I. Some properties of the spheroplast assay system.

Authors:  G Hotz; R Mauser
Journal:  Mol Gen Genet       Date:  1969

Review 6.  Recognition of nucleotide sequences.

Authors:  M Yarus
Journal:  Annu Rev Biochem       Date:  1969       Impact factor: 23.643

7.  DNA repair mechanisms and their relation to mutation and recombination.

Authors:  B S Strauss
Journal:  Curr Top Microbiol Immunol       Date:  1968       Impact factor: 4.291

8.  Serologic estimation of thymine dimers in the deoxyribonucleic acid of bacterial and mammalian cells following irradiation with ultraviolet light and postirradiation repair.

Authors:  E Seaman; H Van Vunakis; L Levine
Journal:  J Biol Chem       Date:  1972-09-25       Impact factor: 5.157

9.  Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light.

Authors:  L Levine; E Seaman; E Hammerschlag; H Van Vunakis
Journal:  Science       Date:  1966-09-30       Impact factor: 47.728
  9 in total
  2 in total

1.  Characteristics of excision repair of pyrimidine dimers in eukaryotic cells as assayed with anti-dimer sera.

Authors:  J J Cornelis
Journal:  Nucleic Acids Res       Date:  1978-11       Impact factor: 16.971

2.  On the immunological detection of X-ray induced DNA damage.

Authors:  H Waller; E Friess; J Kiefer
Journal:  Radiat Environ Biophys       Date:  1981       Impact factor: 1.925
  2 in total

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