Literature DB >> 521318

The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts.

M Graf, U Leeman, F Ruch, P Sträuli.   

Abstract

Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde. With both methods cathepsin B was found in small granules distributed throughout the cytoplasm.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 521318     DOI： 10.1007/BF00495033

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  5 in total

1.  On the substrate specificity of cathepsins B1 and B2 including a new fluorogenic substrate for cathepsin B1.

Authors:  J K McDonald; S Ellis
Journal:  Life Sci       Date:  1975-10-15       Impact factor: 5.037

2.  FIXATION OF TISSUE CULTURE CELLS FOR ULTRASTRUCTURAL CYTOCHEMISTRY.

Authors:  G B GORDON; L R MILLER; K G BENSCH
Journal:  Exp Cell Res       Date:  1963-08       Impact factor: 3.905

Review 3.  The use of amino acid derivatives of 4-methoxy-beta-naphthylamine for the assay and subcellular localization of tissue proteinases.

Authors:  R E Smith; R M van Frank
Journal:  Front Biol       Date:  1975

4.  A new assay for cathepsin B1 and other thiol proteinases.

Authors:  A J Barrett
Journal:  Anal Biochem       Date:  1972-05       Impact factor: 3.365

5.  Flow cytometric measurement of peptidases with use of 5-nitrosalicylaldehyde and 4-methoxy-beta-naphthylamine derivatives.

Authors:  F A Dolbeare; R E Smith
Journal:  Clin Chem       Date:  1977-08       Impact factor: 8.327
  5 in total
  9 in total

1.  Localization of cathepsin B activity in fibroblasts and chondrocytes by continuous monitoring of the formation of a final fluorescent reaction product using 5-nitrosalicylaldehyde.

Authors:  C J Van Noorden; I M Vogels; V Everts; W Beertsen
Journal:  Histochem J       Date:  1987-09

2.  Decreased lysosomal protease content of skeletal muscles from streptozotocin-induced diabetic rats: a biochemical and histochemical study.

Authors:  W T Stauber; V K Fritz
Journal:  Histochem J       Date:  1985-05

3.  Immunological demonstration of intestinal absorption and digestion of protein macromolecules in the trout (Salmo gairdneri).

Authors:  U Georgopoulou; M F Sire; J M Vernier
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

4.  Enzyme histochemical reactions in unfixed and undecalcified cryostat sections of mouse knee joints with special reference to arthritic lesions.

Authors:  C J Van Noorden; I M Vogels
Journal:  Histochemistry       Date:  1986

5.  Tumor cell proteinase visualization and quantification using a fluorescent transition-state analog probe.

Authors:  K A Kozlowski; F H Wezeman; R M Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

6.  Cathepsin B in osteoarthritis: cytochemical and histochemical analysis of human femoral head cartilage.

Authors:  A Baici; A Lang; D Hörler; R Kissling; C Merlin
Journal:  Ann Rheum Dis       Date:  1995-04       Impact factor: 19.103

7.  The identification of the major excreted protein (MEP) from a transformed mouse fibroblast cell line as a catalytically active precursor form of cathepsin L.

Authors:  R W Mason; S Gal; M M Gottesman
Journal:  Biochem J       Date:  1987-12-01       Impact factor: 3.857

8.  High cathepsin B activity in alveolar macrophages occurs with elastase-induced emphysema but not with bleomycin-induced pulmonary fibrosis in hamsters.

Authors:  M L Padilla; N I Galicki; J Kleinerman; M Orlowski; M Lesser
Journal:  Am J Pathol       Date:  1988-04       Impact factor: 4.307

9.  Cathepsin B containing cells in the rabbit mesentery during invasion of V2 carcinoma cells.

Authors:  F M Graf; G Haemmerli; P Sträuli
Journal:  Histochemistry       Date:  1984
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.