Literature DB >> 518557

Photoactivated covalent binding of [3H]bilirubin to human serum albumin.

D W Hutchinson, D S Mutopo.   

Abstract

A one-step procedure has been developed for the preparation of [3H]bilirubin IX-alpha in good yield from unlabelled bilirubin. Irradiation of an aqueous solution of [3H]bilirubin IX-alpha in the presence of human serum albumin results in the covalent attachment of the bilirubin to the protein. Preliminary degradation studies have been carried out to locate the site of attachment of the bilirubin to the albumin.

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Year:  1979        PMID： 518557      PMCID： PMC1161221          DOI： 10.1042/bj1810779

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Commercial bilirubin: A trinity of isomers.

Authors:  A F. McDonagh; F Assisi
Journal:  FEBS Lett       Date:  1971-11-01       Impact factor: 4.124

2.  Affinity labeling of the primary bilirubin binding site of human serum albumin.

Authors:  C C Kuenzle; N Gitzelmann-Cumarasamy; K J Wilson
Journal:  J Biol Chem       Date:  1976-02-10       Impact factor: 5.157

3.  Mechanisms of the photodegradation of bilirubin.

Authors:  R Bonnett
Journal:  Biochem Soc Trans       Date:  1976       Impact factor: 5.407

4.  The 3H-labelling of bilirubin.

Authors:  F E Hancock; D W Hutchinson; A J Knell
Journal:  Biochem J       Date:  1976-08-01       Impact factor: 3.857

5.  Photoaddition of sulphydryl groups to bilirubin in vitro.

Authors:  P Manitto; D Monti
Journal:  Experientia       Date:  1972-04-15

6.  Cyanogen bromide fragments of human serum albumin.

Authors:  R H McMenamy; H M Dintzis; F Watson
Journal:  J Biol Chem       Date:  1971-08-10       Impact factor: 5.157

7.  Removal of fatty acids from serum albumin by charcoal treatment.

Authors:  R F Chen
Journal:  J Biol Chem       Date:  1967-01-25       Impact factor: 5.157

8.  Physical and binding properties of large fragments of human serum albumin.

Authors:  M J Geisow; G H Beaven
Journal:  Biochem J       Date:  1977-06-01       Impact factor: 3.857

9.  The synthesis of phenyl(2-3H)glyoxal.

Authors:  B W Augustus; D W Hutchinson
Journal:  Biochem J       Date:  1979-01-01       Impact factor: 3.857

10.  The reaction between bilirubin and aromatic diazo compounds.

Authors:  D W Hutchinson; B Johnson; A J Knell
Journal:  Biochem J       Date:  1972-05       Impact factor: 3.857
  10 in total
  1 in total

1.  High-pressure liquid chromatographic analysis of anaerobic photoproducts of bilirubin-IX alpha in vitro and its comparison with photoproducts in vivo.

Authors:  S Onishi; N Kawade; S Itoh; K Isobe; S Sugiyama
Journal:  Biochem J       Date:  1980-09-15       Impact factor: 3.857
  1 in total

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