Literature DB >> 518555

Delineation of fucosyltransferase activities with thiol reagents.

D Kessel, T H Chou.   

Abstract

The thiol reagent dithiothreitol inhibits the activity of a core GDP-fucose-N-acetylglucosaminide alpha-6-fucosyltransferase in plasma and blood-cell homogenates, while promoting the activity of alpha-2- and alpha-3-fucosyltransferases. The latter enzymes catalyse transfer of fucose on to terminal galactose and subterminal N-acetylglucosamine residues respectively. A thiol-blocking reagent N-ethylmaleimide does not affect the activity of the alpha-6-fucosyltransferase, but inhibits the other two enzymes. These results indicate the presence of a critical disulphide linkage in the alpha-6-fucosyltransferase, and provide a means of delineation of different fucosyltransferases.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 518555      PMCID： PMC1161218          DOI： 10.1042/bj1810767

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS.

Authors:  W W CLELAND
Journal:  Biochemistry       Date:  1964-04       Impact factor: 3.162

2.  PERIODATE OXIDATION OF THE GLYCOPROTEIN FETUIN.

Authors:  R G SPIRO
Journal:  J Biol Chem       Date:  1964-02       Impact factor: 5.157

3.  Variation of levels of plasma guanosine diphosphate L-fucose:beta-D-galactosyl alpha-2-L-fucosyltransferase in acute adult leukemia.

Authors:  P Khilanani; T H Chou; P L Lomen; D Kessel
Journal:  Cancer Res       Date:  1977-08       Impact factor: 12.701

4.  The control of glycoprotein synthesis: N-acetylglucosamine linkage to a mannose residue as a signal for the attachment of L-fucose to the asparagine-linked N-acetylglucosamine residue of glycopeptide from alpha1-acid glycoprotein.

Authors:  J R Wilson; D Williams; H Schachter
Journal:  Biochem Biophys Res Commun       Date:  1976-10-04       Impact factor: 3.575

5.  Occurrence of two fucosyltransferase activities at the outer surface of rat lymphocytes.

Authors:  B Hoflack; R Cacan; A Verbert
Journal:  Eur J Biochem       Date:  1978-07-17

6.  Blood group gene specified glycosyltransferases in rare ABO groups and in leukaemia.

Authors:  W M Watkins
Journal:  Rev Fr Transfus Immunohematol       Date:  1978-02

7.  Selective inhibition of a plasma fucosyltransferase by N-ethylmaleimide.

Authors:  T H Chou; C Murphy; D Kessel
Journal:  Biochem Biophys Res Commun       Date:  1977-02-07       Impact factor: 3.575

8.  Activity of 2-alpha-L-fucosyltransferase in human sera and red cell membranes. A study of common ABH blood donors, rare 'Bombay' and 'Parabombay' individuals.

Authors:  C Mulet; J P Cartron; J Badet; C Salmon
Journal:  FEBS Lett       Date:  1977-12-01       Impact factor: 4.124

9.  Guanosine diphosphate-L-fucose plasma: N-acetylglucosaminide fucosyltransferase as in index of bone marrow hyperplasia after chemotherapy.

Authors:  P Khilanani; T H Chou; D Kessel
Journal:  Cancer Res       Date:  1978-01       Impact factor: 12.701

10.  Fucosyltransferase activity in metastasizing and nonmetastasizing rat mammary carcinomas.

Authors:  S K Chatterjee; U Kim
Journal:  J Natl Cancer Inst       Date:  1978-07       Impact factor: 13.506
View more
  1 in total

1.  A novel method for determination of alpha1,6fucosyltransferase activity using a reducing oligosaccharide from egg yolk as a specific acceptor.

Authors:  S Yazawa; N Kochibe; T Nishimura; C Shima; I Takai; M Adachi; T Asao; T Hada; Y Enoki; L R Juneja
Journal:  Glycoconj J       Date:  1998-09       Impact factor: 2.916
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.