Literature DB >> 5166859

Purification and characterization of the L component of Streptococcus zymogenes lysin.

P A Granato, R W Jackson.   

Abstract

By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the L component of the group D lysin produced by Streptococcus zymogenes strains has been purified to a state of homogeneity as determined by the techniques of disc-gel electrophoresis at pH 9.3 and 4.3 and isoelectric focusing. The L component was found to be a protein possessing a molecular weight of 11,000 with a slight net negative charge at physiological pH.

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Year:  1971        PMID： 5166859      PMCID： PMC247144          DOI： 10.1128/jb.108.2.804-808.1971

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  7 in total

1.  A rapid method of total lipid extraction and purification.

Authors:  E G BLIGH; W J DYER
Journal:  Can J Biochem Physiol       Date:  1959-08

2.  STARCH-GEL ELECTROPHORESIS--APPLICATION TO THE CLASSIFICATION OF PITUITARY PROTEINS AND POLYPEPTIDES.

Authors:  K A FERGUSON
Journal:  Metabolism       Date:  1964-10       Impact factor: 8.694

3.  The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid.

Authors:  B N AMES; D T DUBIN
Journal:  J Biol Chem       Date:  1960-03       Impact factor: 5.157

4.  Bicomponent nature of lysin from Streptococcus zymogenes.

Authors:  P A Granato; R W Jackson
Journal:  J Bacteriol       Date:  1969-11       Impact factor: 3.490

5.  Characterization of the A component of Streptococcus zymogenes lysin.

Authors:  P A Granato; R W Jackson
Journal:  J Bacteriol       Date:  1971-08       Impact factor: 3.490

6.  Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

Authors:  J L Hedrick; A J Smith
Journal:  Arch Biochem Biophys       Date:  1968-07       Impact factor: 4.013

7.  Use of a logistic function in expressing kinetic hemolysis data.

Authors:  R W Jackson; S F Basinger; J M Werth
Journal:  Infect Immun       Date:  1970-02       Impact factor: 3.441
  7 in total
  9 in total

Review 1.  Colicinogeny and related phenomena.

Authors:  K G Hardy
Journal:  Bacteriol Rev       Date:  1975-12

2.  Production of Hemolysin and Bacteriolysin in a Synthetic Medium by Streptococcus faecalis var. zymogenes.

Authors:  B Appelbaum; L N Zimmerman
Journal:  Infect Immun       Date:  1974-11       Impact factor: 3.441

3.  Molecular characterization of the Enterococcus faecalis cytolysin activator.

Authors:  R A Segarra; M C Booth; D A Morales; M M Huycke; M S Gilmore
Journal:  Infect Immun       Date:  1991-04       Impact factor: 3.441

Review 4.  Movable genetic elements and antibiotic resistance in enterococci.

Authors:  D B Clewell
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1990-02       Impact factor: 3.267

Review 5.  Virulence of enterococci.

Authors:  B D Jett; M M Huycke; M S Gilmore
Journal:  Clin Microbiol Rev       Date:  1994-10       Impact factor: 26.132

6.  An HlyB-type function is required for expression of the Enterococcus faecalis hemolysin/bacteriocin.

Authors:  M S Gilmore; R A Segarra; M C Booth
Journal:  Infect Immun       Date:  1990-12       Impact factor: 3.441

7.  Genetic analysis of the pAD1 hemolysin/bacteriocin determinant in Enterococcus faecalis: Tn917 insertional mutagenesis and cloning.

Authors:  Y Ike; D B Clewell; R A Segarra; M S Gilmore
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

8.  Self-transferable plasmids determining the hemolysin and bacteriocin of Streptococcus faecalis var. zymogenes.

Authors:  A E Jacob; G J Douglas; S J Hobbs
Journal:  J Bacteriol       Date:  1975-03       Impact factor: 3.490

9.  Bacteremia caused by hemolytic, high-level gentamicin-resistant Enterococcus faecalis.

Authors:  M M Huycke; C A Spiegel; M S Gilmore
Journal:  Antimicrob Agents Chemother       Date:  1991-08       Impact factor: 5.191
  9 in total

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