Literature DB >> 5164097

Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase.

S Goldfarb, H C Pitot.   

Abstract

Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-(14)C anhydride and a double-label ((14)C and (3)H) method for determining the amount of mevalonate-3-(14)C that is formed from the substrate.

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Year:  1971        PMID: 5164097

Source DB:  PubMed          Journal:  J Lipid Res        ISSN: 0022-2275            Impact factor:   5.922


  24 in total

1.  Influence of multiple cysteines on human 3-hydroxy-3-methylglutaryl-CoA lyase activity and formation of inter-subunit adducts.

Authors:  Christa Montgomery; Henry M Miziorko
Journal:  Arch Biochem Biophys       Date:  2011-04-13       Impact factor: 4.013

2.  The regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the isolated perfused rat liver.

Authors:  A D Cooper
Journal:  J Clin Invest       Date:  1976-06       Impact factor: 14.808

3.  A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this effect of dietary cholesterol on this enzyme.

Authors:  Y A Baqir; R Booth
Journal:  Biochem J       Date:  1977-06-15       Impact factor: 3.857

4.  Diurnal variations in rat liver 3-hydroxy-3-methylglutaryl-Coenzyme A reductase activity in relation to feeding.

Authors:  K W Gregory; C Z Smith; R Booth
Journal:  Biochem J       Date:  1972-12       Impact factor: 3.857

5.  A cold-clamping technique for the rapid sampling of rat liver for studies on enzymes in separate cell fractions. Suitability for the study of enzymes regulated by reversible phosphorylation-dephosphorylation.

Authors:  R A Easom; V A Zammit
Journal:  Biochem J       Date:  1984-06-15       Impact factor: 3.857

6.  Evidence for post-transcriptional regulation by insulin of 3-hydroxy-3-methylglutaryl coenzyme A reductase and sterol synthesis in human mononuclear leucocytes.

Authors:  W Krone; H Greten
Journal:  Diabetologia       Date:  1984-05       Impact factor: 10.122

7.  Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in rat liver and Morris hepatomas 5123C, 9618A and 5123t.c.

Authors:  R G Gregg; J R Sabine; P A Wilce
Journal:  Biochem J       Date:  1982-05-15       Impact factor: 3.857

8.  Effect of insulin on sterol and fatty acid synthesis and hydroxymethylglutaryl CoA reductase activity in mammalian cells grown in culture.

Authors:  S J Bhathena; J Avigan; M E Schreiner
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

9.  Stimulation of hepatic beta-hydroxy-beta-methylglutaryl coenzyme A reductase activity in hypophysectomized rats by L-triiodothyronine.

Authors:  G C Ness; R E Dugan; M R Lakshmanan; C M Nepokroeff; J W Porter
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

10.  Determination of key intermediates in cholesterol and bile acid biosynthesis by stable isotope dilution mass spectrometry.

Authors:  Tadashi Yoshida; Akira Honda; Hiroshi Miyazaki; Yasushi Matsuzaki
Journal:  Anal Chem Insights       Date:  2008-03-25
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